Johansson L

[ 1 ] - I-9: Activation and Selection of Sperm for IVF and ICSI

Background: During spermatogenesis the sperm nucleus exchange histones against cystein rich protamines, which stabilize the sperm chromatin and protects the DNA against damages. At ejaculation the age of the patient, handling routines, processing of the semen sample and the storage of the final sperm suspension might damage the DNA. Sperm chromatin stability and DNA damages are evaluated with d...

[ 2 ] - I-10: QA in The ART Laboratory

The establishment of a high quality ART program requires a multi-disciplinary effort, involvement and commitment of all staff members - team work. It is of vital importance that all aspects of the treatments are taken into consideration and that you already from the start use international guidelines, standards and indicators to initiate the construction of a well-organised ART program that mee...

[ 3 ] - I-11: Optimizing the ART Outcome

Background: The establishment of a high quality ART program requires a multi-disciplinary effort, involvement and commitment of all staff members – team work. It is of vital importance that all aspects of the treatments are taken into consideration and that you already from the start use high international standards to initiate a wellorganised ART program that meets the requirements from patien...

[ 4 ] - I-12: Physiological ICSI

Prior to ICSI the cumulus-oophorous-complex of the oocyte is commonly remove with impure animal-derived hyaluronidase of low strength. The lower strength of the enzyme, the high concentration of impurities and the necessity of extensive time-dependent mechanical denudation damages of the oocytes, increases the risk of lysing the oocytes and the transfer of animal-derived pathogens. All this can...

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