Ali Reza Saeedinia

[ 1 ] - The modified recombinant proinsulin: a simple and efficient route to produce insulin glargine in E. coli

Background: Recombinant insulin glargine, a long-acting analogue of insulin, is expressed as proinsulin in host cell and after purification and refolding steps cleaved to active insulin by enzymatic digestion using trypsin and carboxypeptidase B. Since the proinsulin's B and C chains have several internal arginine and lysine residues, a number of impurities are generated following treatment wit...