Ahmad Reza Jabbari
Pasteurella National Research Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization,Karaj, Iran
[ 1 ] - LPS-PCR typing of ovine Pasteurella multocida isolates from Iran based on (L1 to L8) outer core biosynthesis loci
Pasteurella multocida isa gram-negative bacterial pathogen that is causative agent of a wide range of diseases in many animal species and humans. Lipopolysaccharides (LPS) are an important virulence factor, minor changes to structure of which can exert dramatic effects on pathogenicity of P. multocida in its host. LPS can be used for the identification and classification of strains with somatic...
[ 2 ] - Prevalence of adhesion Virulence factor genes, antibiogram, and pathogenicity of avian Pasteurella multocida isolate from Iran
Pasteurella multocida possesses various virulence factors, including capsule, lipopolysaccharide, fimbriae, toxins, outer membrane proteins, and adhesions. Adhesins have a crucial role in mediating colonization and invasion of the host. The aim of the present study was to identify the prevalence of adhesion factor genes and resistance/sensitivity patterns among the avian P. multocida isolates f...
[ 3 ] - Isolation, identification, and monitoring of antibiotic resistance in Pasteurella multocida and Mannheimia haemolytica isolated from sheep in East Azerbaijan province, Iran
The present study was carried out in order to isolate, identify, and assess the antimicrobial susceptibility of the causative agent(s) of pneumonic pasteurellosis in sheep in East Azerbaijan province, northwest of Iran. Pneumonia was detected in 320 cases, and the affected lungs were sampled in the slaughterhouse. The samples were investigated bacteriologically for the isolation of two microorg...
[ 4 ] - Genotyping of Pasteurella multocida ovine and bovine isolates from Iran based on PCR-RFLP of ompH gene
Pasteurella multocida (P. multocida), A Gram-negative facultative anaerobic bacterium, is a causative animal pathogen in porcine atrophic rhinitis and avian fowl cholera. The outer membrane of Gram-negative bacteria contains of many different protein in very high copy numbers. One of the major outer membrane, the H proteins have functional as high immunogenicity and antigenicity. In this study ...
[ 5 ] - Isolation and Identification of Pasteurella multocida from Sheep & Goat in Iran
This study has been carried out with the objective of isolation and identification of agent(s) of pasteurella pneumonia in sheep and goat in Iran using bacteriological and biochemical assays to be identified in the pursuant researches to be used in pasteurellosis vaccine production. To accomplish this objective, samples were gathered from areas suspicious to pasteurellosis infection and industr...
[ 6 ] - Occurrence of Beta2 toxigenic Clostridium perfringens isolates with different toxin types in Iran
Clostridium perfringens is an important cause of enteric diseases in both human and animals. The bacteria produce several toxins which play key roles in the pathogenesis of diseases and are classified into five toxin types, on the basis of the differential production of Alpha, Beta, Epsilon and Iota toxins. In this study a single PCR assay was developed and used for detection of cpb2 gene to id...
[ 7 ] - Large scale production of Blackleg vaccine by fermenter and enriched culture medium in Iran
In all biological systems growth is defined as increase of chemical compounds. Bacteria can achieve to balanced growth if they are growing in a medium, which are completely adapted to it. Clostridium chauvoei, (Clostridium feseri) is an anaerobic, spore forming, motile, and polymorph bacteria, which its size varies from 0.5-1 to 3-8 micron and could be observed as individual bacterium, diplo, a...
[ 8 ] - Molecular typing of toxigenic Clostridum perfringens isolated from sheep in Iran
In this research a molecular method based on polymerase chain reaction for typing of Clostridium perfringens was developed and toxin genotypes of 64 isolates from sheep and goats in Iran were determined. The PCR assays were developed for detection of alpha (cpa), beta (cpb) and epsilon (etx) toxin genes, allowing classification of the isolates into genotypes A B, C and D. The field isolates ...
[ 9 ] - Fusion of Clostridium perfringens type D and B epsilon and beta toxin genes and it’s cloning in E. coli
Designing and producing a proper fusion construction is the most important problem of producing large quantities of a properly folded functional protein. This construction should have all necessary components of a real gene. A good designed fusion gene construction could be cloned into a good and suitable host. Clostridium perfringens is an important pathogen of humans and livestock and produce...
[ 10 ] - Experimintal Study of Peracute Fowl Cholera due to Pasteurella multocida Vaccinal Strain (serotype A1) in Chickens
In order to show the type and severity of gross and histopathologic lesions induce by vaccinal strain (serotype A1) of Pateurella mulocida, ten four-week-old SPF chickens were inoculated intramuscularly with 75 cfu of ( 0.5 ml of 10-7 dilution) bacterium. All birds died in less than 16 hours. No prominent gross lesions were observed in different organs. In microscopic examination, the most comm...
[ 11 ] - Evaluation the Efficacy of Anthrax Vaccine against Challenge with a Highly Virulent Strain of Bacillus anthracis Isolated from Soil in Sheep, Goats and Guinea Pigs in Iran
Protection of animals immunized against Bacillus anthracis is usually demonstrated by challenging with an appropriate dose of a strain of Bacillus anthracis that is lethal to unvaccinated animals inoculated at the same time. In this study the protective efficacy in anthrax vaccine (34F2 sterne strain spore) was evaluated in sheep, goats and guinea pigs challenged with subcutaneous inoculation...
[ 13 ] - Isolation of Anthrax Spores from Soil in Endemic Regions of Isfahan, Iran
To isolate and detect anthrax spores from soil in different regions of Isfahan, Iran a total of 60 environmental specimens were collected during 2003. Bacterial endospores were extracted via flotation in distilled water and were cultured on blood agar and selective PLET media. Bacillus anthracis was identified using bacteriological and biological tests. Viable Bacillus anthracis spores were iso...
[ 14 ] - Pulmonary Aspergillosis in Ostrich
[ 17 ] - Distribution of virulence adhesion associated genes and antimicrobial susceptibility in Pasteurella multocida from ovine pasteurellosis in Iran
Pasteurella multocida is an opportunistic pathogen responsible for pneumonia of lambs and goats, respiratory atrophic rhinitis of swine. Although the molecular basis of the pathogenicity and host specificity of P. multocida is not well understood, several studies have reported that a number of proteins are correlated with the pathogenic mechanisms. Adhesins have a crucial role in mediating colo...
[ 18 ] - Identification of enterototxin harboring gene among Clostridium perfringens isolates with different toxin types in Iran
Background: Clostridium perfringens is known as the most widely distributed pathogenic microorganism in nature. It is an extremely important pathogen of human and domestic animals. In a commonly used classification scheme, C. perfringens is divided into five toxinotypes (A to E) based on the production of four major toxins (alpha, beta, epsilon, and iota). Enterotoxin is not usually used for C....
[ 19 ] - Sequencing and comparative analysis of flagellin genes fliA and fliB in bovine Clostridium chauvoei isolates
Background: Clostridium chauvoei is the etiological agent of blackleg as an endogenous infection in cattle. Flagella have been known to play a critical role in the protective immunity of animals to clostridial infections. C.chauvoei has two copies of fliC gene, namely fliA and fliB. OBJECTIVES: The aim of this study was the determination and nucleotide sequence analysis of both copies of fliC ...
[ 20 ] - تولید واکسن براکسی با استفاده از محیط کشت غنی شده و فرمانتور
زمینه مطالعه: واکسن براکسی عبارتست از کشت سویه مناسبی از کلستریدیوم سپتیکوم در یک محیط مایع و یا فیلترات آن کشت و یا مشتقات آن که به نحوی غیرفعال شده و سمیت آن از بین رفته ولی ایمنی زائی آن برقرار مانده باشد. هدف: تولید واکسن براکسی با استفاده از محیط کشت غنی شده در فرمانتور. روش کار: دو محیط کشت مرسوم و غنی شده برای کشت کلستریدیوم سپتیکوم سویه» 913CNدر ظروف شیشه ای 10 لیتری و در فرما...
[ 21 ] - شناسایی سویههای واکسن گاومیش و پرندگان پاستورلا مولتوسیدا ساخت موسسه رازی بر مبنای نسخه استرالیا طبقهبندی ژنتیکی به روش آنالیز توالی نوکلئوتیدها در لوکوسهای چندگانه
سپتی سمی همراه با خونریزی در اساس یک بیماری عفونی دستگاه تنفسی در نشخوارکنندگان و پرندگان میباشد که توسط پاستورلا مولتوسیدا ایجاد و منجر به خسارتهای اقتصادی قابل توجه میگردد. در میانه سالهای دهه 1930 و در ابتدای دهه 1980 میلادی سویههای ایرانی گاومیش (Pm Razi0001) و پرندگان (Pm Razi0002) پاستورلا مولتوسیدا از حیوانات بیمار جداسازی و تاکنون از آنها در تولید واکسن پاستورلوز موسسه رازی استفاده...
[ 22 ] - جداسازی و شناسایی میکروبیولوژیک جدایههای پاستورلا مولتوسیدا از گاو در کشتارگاههای البرز و تهران
این مطالعه کشتارگاهی با هدف بررسی حضور پاستورلا مولتوسیدا، جمعآوری جدایههای آن و همچنین تعیین تیپ کپسولی آنها در یک واحد صنعتی کشتارگاهی در استان البرز (شهرستان کرج) و 5 واحد در استان تهران (رباط کریم، شهر ری، شهریار، کهریزک و ورامین) و انجام پذیرفت. تعداد 89 نمونه ریه و 595 سواب از مجاری تنفسی گوسالههای گاو در یک کشتارگاه در کرج و 5 کشتارگاه در تهران بر روی محیطهای اختصاصی کشت گردیدند. د...
[ 23 ] - بررسی اپیدمیولوژی مولکولی جدایههای گاوی گوسفندی و بزی مایکوباکتریوم پاراتوبرکلوزیس موجود در بانک میکروبی موسسه تحقیقات واکسن و سرمسازی رازی با استفاده از روش
مایکوباکتریوم آویوم زیرگونه پاراتوبرکولوزیس (MAP) در نشخوارکنندگان عامل ایجاد پاراتوبرکولوزیس میباشد که بدلیل تحمیل خسارتهای اقتصادی گسترده از اهمیت اقتصادی زیاد برخوردار میباشد. در سالهای اخیر استفاده از توالیهای تکراری کوتاه در ژنوتایپینگ این باکتری مورد توجه قرار گرفته است. در مطالعه حاضر نتایج حاصل از کاربرد این روش با اتکا بر روی 2 لوکوس SSR8 و SSR9 بر روی 3 جدایه کلینیکی و 1 سویه مرج...
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