Jafar Akbari
Department of Chemistry, Faculty of Science, Tarbiat Modares University, Tehran, Iran
[ 1 ] - Simple and Rapid Immobilization of Firefly Luciferase on Functionalized Magnetic Nanoparticles; a Try to Improve Kinetic Properties and Stability
We expressed and purified a recombinant P. pyralis luciferase with N-terminal His-tags. The silanized Ni or Cu-loaded magnetic particles were prepared and used to assemble the His-tagged P. pyralis luciferase. This enzyme immobilized on functionalized magnetic nanoparticles (MNPs) via electrostatic interactions of His-tag with Ni2+/Cu2+ ions on the surface of MNPs using si...
نویسندگان همکار