Single nucleotide polymorphisms (SNPs) are single base pair mutations that provide new approaches to studies of allele transcript abundances. High-resolution DNA melting curve (HRM) analysis using a LightScanner (Hi-Res Melting system with Idaho's LC Green) provides post-PCR detection of mutations and SNPs in genomic DNA. This study investigated whether the HRM analysis can distinguish alleles ...

BACKGROUND Microsatellite instability (MSI) is an important marker for screening for hereditary nonpolyposis colorectal cancer (Lynch syndrome) as well as a prognostic and predictive marker for sporadic colorectal cancer (CRC). The mononucleotide microsatellite marker panel is a well-established and superior alternative to the traditional Bethesda MSI analysis panel, and does not require testin...

We have developed a single nucleotide polymorphism (SNP) nucleated high-resolution melting (HRM) technique to genotype Enterococcus faecium. Eight SNPs were derived from the E. faecium multilocus sequence typing (MLST) database and amplified fragments containing these SNPs were interrogated by HRM. We tested the HRM genotyping scheme on 85 E. faecium bloodstream isolates and compared the result...

OBJECTIVE To investigate whether an accreditation program facilitates healthcare organizations (HCOs) to evolve and maintain high-performance human resource management (HRM) systems. DESIGN Cross-sectional multimethod study. SETTING AND PARTICIPANTS Healthcare organizations participating in the Australian Council on Healthcare Standards Evaluation and Quality Improvement Program (EQuIP 4) b...

The relationship between organisations' value system and their human resource management (HRM) system is investigated surveying human resource managers in 443 Australian organisations. Organisations were grouped into four main value types Elite, Leadership, Meritocratic and Collegial, according to a typological theory of organisational values (Kabanoff, 1991a,b, 1993). MANOVA and discriminant a...

submitted to the DRUID Summer conference 2003 on Creating, Sharing, and Transferring Knowledge: The role of Geography, Institutions, Organisations. The proposed paper in related to conference themes B and E. Exploiting old and new knowledge in combination in order to generate new knowledge is a key feature in achieving competitive advantage of the firm. The emergence of the knowledgebased econo...

Acknowledgements Material from the Workplace Employee Relations Survey (2004) has been made available by the Office of National Statistics (ONS) through the UK Data Archive. We would also like to acknowledge the financial assistance of BERR. None of these bodies is responsible for the views expressed in the paper. The views expressed in this report are the authors' and do not necessarily reflec...

Radiofrequency catheter ablation (RFCA) is a potentially curative method for treatment of highly symptomatic and drug-refractory atrial fibrillation (AF). However, this technique can provoke esophageal and nerve lesion, due to thermal injury. To our knowledge, there have been no reported cases of a newly described motor disorder, the Jackhammer esophagus (JE) after RFCA, independently of GERD. ...

High resolution melting (HRM) is a simple and rapid method for screening mutations. It offers various advantages for clinical diagnostic applications. Conventional HRM analysis often yields equivocal results, especially for surgically obtained tissues. We attempted to improve HRM analyses for more effective applications to clinical diagnostics. HRM analyses were performed for IDH1R132 and IDH2R...

Osteogenesis imperfecta (OI), a congenital bone disorder, is caused by mutations in COL1A1 and COL1A2 genes, leading to deficiency of type I collagen. The high resolution melting (HRM) analysis has been used for detecting mutations, polymorphisms and epigenetic alteration in double-stranded DNAs. This study was to evaluate the potential application of HRM analysis for identifying gene mutations...