OBJECTIVE The main objective of this study was to comparatively evaluate the performance of M.I.C.E. and Etest methodologies to that of agar dilution for determining the antimicrobial susceptibility profile of oxacillin-resistant Staphylococcus spp. METHODS A total of 100 oxacillin-resistant Staphylococcus spp. isolates were collected from hospitalized patients at a teaching hospital. Antimic...

OBJECTIVES To evaluate the suitability of disc diffusion (DD) assay for testing posaconazole activity and to corroborate its activity against recently isolated yeasts by the CLSI reference microdilution M27-A2 method. METHODS A total of 224 yeast isolates (7 species with 52 to 11 isolates each, and 15 species with 1 to 6 isolates) were evaluated, 125 were recent bloodstream isolates, 30 isola...

The commercial technique Vitek 2 system for antifungal susceptibility testing of yeast species was evaluated. A collection of 154 clinical yeast isolates, including amphotericin B- and azole-resistant organisms, was tested. Results were compared with those obtained by the reference procedures of both the CLSI and the European Committee on Antimicrobial Susceptibility Testing (EUCAST). Two other...

BACKGROUND The mortality of Candida Bloodstream Infection (CBSI) remains high. Antifungal susceptibility breakpoints were recently updated for Candida species, the impact remains unknown. In this study we evaluated the impact of inappropriate antifungal treatment according to recent breakpoints on 30-day mortality of CBSI. METHODS From June 2008 to July 2014, data on CBSI episodes from two te...

Swab transport systems are used for a variety of specimen types and must maintain organism viability throughout the transport process. The Copan ESwab is a new nylon-flocked swab designed to optimize specimen collection and to minimize entrapment of the specimen. We used the quantitative elution method with recommended strains, as described in CLSI document M40-A, to evaluate the ESwab for main...

Sir, Fluoroquinolones are broad-spectrum antibacterial agents commonly used in clinical practice. When quinolones became widely used, bacterial resistance to them emerged rapidly and, over the past three decades, resistance has continued to increase. In Gram-negative bacteria, quinolone resistance is due primarily to mutations in chromosomal genes encoding quinolone targets DNA gyrase and topoi...

The in vitro activity of isavuconazole against Mucorales isolates measured by EUCAST E.Def 9.2 and CLSI M38-A2 methodologies was investigated in comparison with those of amphotericin B, posaconazole, and voriconazole. Seventy-two isolates were included: 12 of Lichtheimia corymbifera, 5 of Lichtheimia ramosa, 5 of group I and 9 of group II of Mucor circinelloides, 9 of Rhizomucor pusillus, 26 of...

This study validated the robust performances of recently proposed comprehensive landslide susceptibility index model (CLSI) for mapping (LSM) by comparing it to logistic regression (LR) and analytical hierarchy process information value (AHPIV) model. Zhushan County in China, with 373 landslides identified, was used as area. Eight conditioning factors (lithology, slope structure, angle, altitud...

In recent years, antimicrobial resistance has been on the rise and infectious disease specialists other clinicians face several challenges when treating patients with resistant infections. Clinical Laboratory Standards Institute (CLSI) annually publishes guidelines for tests, including revised breakpoints, because of in changes to nomenclature owing advances whole genome sequencing technology. ...

INTRODUCTION The aim of this study was to investigate the effectiveness of ceftaroline against agents frequently isolated from respiratory tract and wound infections. METHODOLOGY The study included a total of 250 strains isolated from various clinical specimens, among which were Streptococcus pyogenes, Streptococcus agalactiae, Streptococcus dysagalactiae, Streptococcus pneumoniae, Haemophilu...