• Here we present an N-terminal derivatization approach, using TMPP-Ac-Osu that improves the HPLC performance for small hydrophilic phosphopeptides. • This approach is compatible with LC/MS and LC/MALDI. • The chromatographic enhancement was evaluated by on-line nanoscale HPLC coupled with Electrospray on a Q-Tof PremierTM. • LC-MALDI/MS/MS analysis was also investigated. Both MS/MS on a Q-Tof ...

MALDI-TOF-MS is a soft ionization technique that has the potential to determine the molecular weight for various compounds, particularly for large biomolecules.1-3 Recently, considerable interest has been focused on the analysis of synthetic polymers by MALDI-MS.4 Compared with the traditional methods of polymer analysis, MALDI-MS can provide more abundant information on molecular weight, repea...

The identification of proteins involved in tumour progression or which permit enhanced or novel therapeutic targeting is essential for cancer research. Direct MALDI analysis of tissue sections is rapidly demonstrating its potential for protein imaging and profiling in the investigation of a range of disease states including cancer. MALDI-mass spectrometry imaging (MALDI-MSI) has been used here ...

MALDI MSI has been recently applied as an innovative tool for detection of molecular distribution within a specific tissue. MALDI MSI requires deposition of an organic compound, known as matrix, on the tissue of interest to assist analyte desorption and ionization, in which the matrix crystal homogeneity and size greatly influence the imaging reproducibility and spatial resolution in MALDI MSI....

Quantitative analysis with mass spectrometry (MS) is important but challenging. Matrix-assisted laser desorption/ionization (MALDI) coupled with time-of-flight (TOF) MS offers superior sensitivity, resolution and speed, but such techniques have numerous disadvantages that hinder quantitative analyses. This review summarizes essential obstacles to analyte quantification with MALDI-TOF MS, includ...

In this study, the previously reported integrated selective enrichment target (ISET)-MALDI-MS analysis was implemented with ssDNA aptamer to address the specific capturing of thrombin in complex samples. By applying a thrombin specific aptamer linked to ISET-MALDI-MS detection, a proof of concept of antibody fragment background reduction in the ISET-MALDI-MS readout is presented. Detection sens...

We evaluated the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the rapid identification of yeast species. Using Bruker Daltonics MALDI BioTyper software, we created a spectral database library with m/z ratios of 2,000 to 20,000 Da for 109 type and reference strains of yeast (44 species in 8 genera). The database was tested for accuracy by...

introduction: tuberculosis (tb) remains a deadly infectious disease despite all the efforts to reduce its incidence. spread of multidrug resistant tb has seriously undermined the efforts to control the disease globally. in this study protein expression profile of mdr and sensitive isolates of mtb were analyzed and compared in order to identify proteins, which could be used in prevention, diagno...

Accurate and defendable taxonomic identification of microalgae strains is vital for culture collections, industry and academia; particularly when addressing issues of intellectual property. We demonstrate the remarkable effectiveness of Matrix Assisted Laser Desorption Ionisation Time of Flight Mass Spectrometry (MALDI-TOF-MS) biotyping to deliver rapid and accurate strain separation, even in s...

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-Tof-MS) has recently become a popular and versatile method to analyze macromolecules from biological origin. In this paper, we will review the application of MALDI-Tof-MS in clinical chemistry and biology. MALDI-Tof-MS is used in clinical chemistry, e.g. disease markers can be identified with MALDI-MS analysis i...