Quantitative immunoelectrophoresis allows a polyvalent approach to immuno-chemical problems, i.e., identification, quantitation, and thereby standardization of antigens and antibodies in complex mixtures, for example as in crude extracts of microorganisms and their corresponding antisera. This approach is a short cut to conventional standardization of single purified substances, since the polyv...

waldenstrom's macroglobulinemia was studied in a 46 year old iranian male with anemia, bleeding, ecchymose and splenomegaly. the diagnosis of macroglobulinemia was established by the presence of veryhigh level of igm paraprotein in sera which was detectable by imrnunoelectrophoresis and rocket immunoelectrophoresis, and by the presence of bone marrow infiltration of plasmoc ytes. family studies...

Hepatitis associated antigen may be detected quickly and reliably by the latex agglutination test, using antiserum from guinea pigs immunized with the antigen. The latex test has a sensitivity comparable to the counter current immunoelectrophoresis technique.

Streptococcal group A, B, and C carbohydrates were analyzed by counterimmunoelectrophoresis, immunoelectrophoresis, and inhibition of immunoprecipitation. Extracts of streptococci group A or C were shown by counterimmunoelectrophoresis to contain both anodic and cathodic migrating components. In immunoelectrophoresis, group A and C substances formed a continuous precipitation line stretching fr...

Immunological methods are being used to determine human chorionic gonadotropin (HCG) in urine and serum during pregnancy (1-4) and also to localize it in tissues and tumors (5). The antisera used in these studies (1-5) were produced by immunizing rabbits with HCG preparations of widely varying specific activities. The HCG-HCG antisera characterization studies that were performed primarily with ...

IWASA, M., YoKOI, T., HIRATA, K. and SAGISAKA, K. Immunoelectrophoretic Analyses of Common Antigens between Human and Monkey Sera. Tohoku J. exp. Med., 1984, 143(1), 117-124 To examine common antigens between human and monkey sera, sera from 19 kinds of monkey were investigated with crossed and rocket immunoelectrophoresis using antisera to human serum proteins. In the crossed immunoelectrophor...

The Pseudomonas aeruginosa polyvalent vaccine PEV and its 16 constituent monovalent extracts from International Antigenic Typing System serotypes 1 through 13 and 15 through 17 (J. J. Miler, J. F. Spilsbury, R. J. Jones, E. A. Roe, and E. J. L. Lowbury, J. Med. Microbiol. 10:19-27, 1977) were subjected to biochemical analysis and to detailed immunochemical analysis with rabbit anti-PEV immunogl...