PURPOSE We tested for protection of blue light-exposed A2E-containing retinal pigment epithelial (RPE) from damage through the implementation of polycarbonate filters containing varying levels of a pigment that absorbs short wavelength light. METHODS Human adult RPE cells (ARPE-19) that had accumulated synthesized A2E were exposed to either a light line delivered from a tungsten halogen sourc...

UNLABELLED There are several age-related microscopic changes in the salivary glands, including the increase in the number of duct-like structures (DLS). However, the true origin and the phenotype of the DLS are not known. OBJECTIVE To evaluate the phenotype and the cell proliferation index of the DLS of human sublingual glands. MATERIAL AND METHODS Sixty sublingual glands obtained from huma...

COMPENSATORY GROWTH IN DOUBLE MUSCLED BULLS 1 2 3 Different Periods of Feed Restriction Before Compensatory Growth in Belgian Blue Bulls: 4 II. Plasma Metabolites and Hormones 5 6 J. L. Hornick, C. Van Eenaeme, M. Diez, V. Minet, and L. Istasse. 7 8 9 10 Department of Nutrition, Veterinary Faculty, Sart Tilman 11 B43 4000 Liège, Belgium 12 13 Phone: 32-(0)4-3664139 14 Fax: 32-(0)4-3664122 15 E-...

Staining of mast cells (MCs), including porcine ones, is critically dependent upon the fixation and staining technique. In the pig, mucosal and submucosal MCs do not stain or stain only faintly after formalin fixation. Some fixation methods are particularly recommended for MC staining, for example the fixation with Carnoy or lead salts. Zinc salt fixation (ZSF) has been reported to work excelle...

A specific feature of anthraquinone dyes (AD) is to mimic the adenine nucleotides ATP, ADP, NAD and NADH, enabling them to act as ligands in interaction with nucleotide-binding sites of several enzymes and receptors. In the present study, the interactions and/or inhibitory effects of eight AD, including Cibacron Blue 3G-A (Reactive Blue 2), Procion Blue MX-R (Reactive Blue 4) and Remazol Brilli...

PURPOSE To evaluate and compare the effects of the following dyes on human pigmented epithelial cells: indocyanine green (ICG), infracyanine green (IfCG), trypan blue (TB), bromophenol blue (BrB), patent blue (PB), and Brilliant Blue G (BBG). METHODS ARPE-19 cells cultured in vitro were exposed to these dyes, and acute and chronic toxicity were evaluated. Cell viability was measured by colori...

UNLABELLED Multicentric or multifocal breast cancer is considered as one of the limitations for sentinel lymph node (SLN) localization. We did a retrospective analysis to evaluate the success rate, sensitivity, accuracy, and negative predictive values of SLN localization in multicentric or multifocal breast lesions. METHODS Fifty-nine patients with multifocal or multicentric breast lesions pr...