نتایج جستجو برای: روش nasba
تعداد نتایج: 369774 فیلتر نتایج به سال:
مقدمه: با توجه به محدودیت های برخی از روش های سرولوژیک در تشخیص افراد دارای عفونت با ویروس های hiv-1 و hcv، استفاده از روش های تشخیصی ملکولی در شناسایی این دو عامل اهمیت بسیار زیادی دارد. با این حال اشکال عمده ی روش های ملکولی هزینه بر بودن آن ها و نیاز به وجود دستگاه ترموسایکلرکه گران قیمت است. روش ها: این پژوهش به راه اندازی و به کارگیری یک روش nasba (nucleic acid sequence based amplification...
We demonstrate the first integrated microfluidic tmRNA purification and nucleic acid sequence-based amplification (NASBA) device incorporating real-time detection. The real-time amplification and detection step produces pathogen-specific response in < 3 min from the chip-purified RNA from 100 lysed bacteria. On-chip RNA purification uses a new silica bead immobilization method. On-chip amplific...
Currently available human immunodeficiency virus type 1 (HIV-1) RNA quantification assays can detect most viruses of the group M subtypes, but a substantial number are missed or not quantified reliably. Viruses of HIV-1 group O cannot be detected by any commercially available assay. We developed and evaluated a quantitative assay based on nucleic acid sequence-based amplification (NASBA) techno...
Accurate quantification of multidrug resistance-1 gene (MDR1) expression in target cells would be of important therapeutic value in predicting cellular response to anticancer drugs. Because certain normal cells in peripheral blood physiologically express MDR1, increasing the sensitivity of the detection methods might result in confounding low-degree expression in tumor cells with physiologic ex...
BACKGROUND The aim of this study was the rapid identification of bla KPC gene in 38 Klebsiella pneumoniae clinical isolates with reduced susceptibility to carbapenems. The modified Hodge Test (MHT) was carried out to phenotypically determine whether resistance to carbapenems was mediated by a carbapenemase. The detection of the bla KPC gene was performed by real-time acid nucleic sequence-based...
The human CCR5 chemokine receptor functions as a coreceptor with CD4 for infection by macrophage-tropic isolates of human immunodeficiency virus type 1 (HIV-1). A mutated CCR5 allele which encodes a protein that does not function as a coreceptor for HIV-1 has been identified. Thus, expression of the wild-type and/or mutation allele is relevant to determining the infectability of patient periphe...
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