RATIONALE We previously discovered the human 10T-->C (Trp4Arg) missense mutation in exon 2 of the muscle LIM protein (MLP, CSRP3) gene. OBJECTIVE We sought to study the effects of this single-nucleotide polymorphism in the in vivo situation. METHODS AND RESULTS We now report the generation and detailed analysis of the corresponding Mlp(W4R/+) and Mlp(W4R/W4R) knock-in animals, which develop...

In this paper, we study a natural extension of multi-layer perceptrons (MLP) to functional inputs. We show that fundamental results for classical MLP can be extended to functional MLP. We obtain universal approximation results that show the expressive power of functional MLP is comparable to that of numerical MLP. We obtain consistency results, which imply that the estimation of optimal paramet...

The basic concept of multi-dimensional limiting process (MLP) on unstructured grids is inherited and modified for improving shock stabilities and reducing numerical dissipation on smooth regions. A relaxed version of MLP condition, simply named as weak-MLP, is proposed for reducing dissipation. Moreover, a stricter condition, that is the strict-MLP condition, is proposed to enhance the numerica...

We deene a Gamma multi-layer perceptron (MLP) as an MLP with the usual synaptic weights replaced by gamma lters (as proposed by de Vries and Principe (de Vries & Principe 1992)) and associated gain terms throughout all layers. We derive gradient descent update equations and apply the model to the recognition of speech phonemes. We nd that both the inclusion of gamma lters in all layers, and the...

Prolonged hemodynamic overload results in cardiac hypertrophy and failure with detrimental changes in myocardial gene expression and morphology. Cysteine-rich protein 3 or muscle LIM protein (MLP) is thought to be a mechanosensor in cardiac myocytes. Therefore, the subcellular location of MLP may have functional implications in health and disease. Our hypothesis is that MLP becomes mislocalized...

Previous work has shown that mutations in muscle LIM protein (MLP) can cause hypertrophic cardiomyopathy (HCM). In order to gain an insight into the molecular basis of the disease phenotype, we analysed the binding characteristics of wild-type MLP and of the (C58G) mutant MLP that causes hypertrophic cardiomyopathy. We show that MLP can form a ternary complex with two of its previously document...