Three dimensional (3D) hydrogel platforms are powerful tools, providing controllable, physiologically relevant microenvironments that could aid in understanding how various environmental factors direct valvular interstitial cell (VIC) phenotype. Continuous activation of VICs and their transformation from quiescent fibroblast to activated myofibroblast phenotype is considered to be an initiating...

01-P007 Characterising microRNA expression and function during embryonic gonadal development Stephanie Bannister, Henk Buermans, Ivano Broz, Mark Tizard, Timothy Doran, Andrew Sinclair, Craig Smith Livestock Industries, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Australian Animal Health Laboratory, Geelong, Vic., Australia Center for Human and Clinical Genetics, Leide...

Eleanor C Saunders, David P De Souza, James I MacRae, Vladimir A Likic’ & Malcolm J McConville 1 Department of Biochemistry and Molecular Biology, Bio21 Institute of Molecular Science and Biotechnology, University of Melbourne, 30 Flemington Road, Parkville, VIC 3010 2 Metabolomics Australia, Bio21 Institute of Molecular Science and Biotechnology, University of Melbourne, 30 Flemington Road, Pa...

IMPROVING EFFICIENCY OF SERVICEORIENTED CONTEXT-DRIVEN SOFTWARE AGENTS Kutila Gunasekera a , Seng Wai Loke b , Arkady Zaslavsky c & Shonali Krishnaswamy a a Faculty of Information Technology, Monash University, Caulfield East, VIC, Australia b Department of Computer Science & Computer Engineering, La Trobe University, Bundoora, VIC, Australia c Faculty of Information Technology, Monash Universi...

01-P007 Characterising microRNA expression and function during embryonic gonadal development Stephanie Bannister, Henk Buermans, Ivano Broz, Mark Tizard, Timothy Doran, Andrew Sinclair, Craig Smith Livestock Industries, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Australian Animal Health Laboratory, Geelong, Vic., Australia Center for Human and Clinical Genetics, Leide...

The safety and efficacy of three-layer (3L) tubular bandaging as a treatment for venous ulcer healing has not been evaluated despite its use in many clinical settings to treat people with venous leg ulcers. We evaluated the safety and efficacy of 3L tubular bandage compared with short-stretch compression bandage to heal venous ulcers in a multicenter, open-label, parallel-group, randomized cont...

We have characterized a recently established mouse pheochromocytoma cell line (MPC 9/3L) as a useful model for studying neurotransmitter release and neuroendocrine secretion. MPC 9/3L cells express many of the proteins involved in Ca2+-dependent neurotransmitter release but do not express functional endogenous Ca2+-influx pathways. When transfected with recombinant N-type Ca2+ channel subunits ...

In this paper, we extend the vehicle routing problem with clustered backhauls (VRPCB) to an integrated routing and three-dimensional loading problem, called VRPCB with 3D loading constraints (3L-VRPCB). In the VRPCB each customer is either a linehaul or a backhaul customer and in each route all linehaul customers must be visited before any backhaul customer. In the 3L-VRPCB, each customer deman...

The vic two-component signal transduction system of Streptococcus pneumoniae is essential for growth. The vic operon comprises three genes encoding the following: VicR, a response regulator of the OmpR family; VicK, its cognate histidine kinase; and VicX, a putative protein sharing 55% identity to the predicted product (YycJ) of an open reading frame in the Bacillus subtilis genome. We show tha...

The C-terminal domains of Dnmt3a and Dnmt3L form elongated heterotetramers (3L-3a-3a-3L). Analytical ultracentrifugation confirmed the Dnmt3a-C/3L-C complex exists as a 2:2 heterotetramer in solution. The 3a-3a interface is the DNA-binding site, while both interfaces are essential for AdoMet binding and catalytic activity. Hairpin bisulfite analysis shows correlated methylation of two CG sites ...