To quantify gene expressions by quantitative reverse transcription-PCR (Q-RT-PCR) in natural diatom assemblages, it is necessary to seek a biomass reference specific to the target species. Two housekeeping genes, TBP (encoding the TATA box-binding protein) and EFL (encoding the translation elongation factor-like protein), were evaluated as candidates for reference genes in Q-RT-PCR assays. Tran...

Marine microalgae have been prominently featured in cancer research. Here, we examined cytotoxic effect and apoptosis mechanism of crude ethanol extracts of an indigenous microalga, Chaetoceros calcitrans (UPMAAHU10) on human breast cell lines. MCF-7 was more sensitive than MCF-10A with IC50 value of 3.00 ± 0.65, whilst the IC50 value of Tamoxifen against MCF-7 was 12.00 ± 0.52  μg/mL after 24 ...

The peroxisome proliferator-activated receptors (PPARs) function as ligand-activated transcription factors that convert signals in the form of lipids to physiological responses through the activation of metabolic target genes. Due to their key roles in lipid and carbohydrate metabolism, the PPARs are important drug targets. However, for several of the PPAR drugs currently in use, adverse side e...

Abstract Although phytoplankton of the Banda Sea has been studied since 1970s, its information is still limited. In order to provide a better understanding diversity, during Expedition Jala Citra 2 2022, sampling was done in surface waters at five stations northern Sea. The purpose this study analyze abundance and species composition southeast monsoon season. Phytoplankton samples were taken di...

In recent years, the role of lectins in feed selection bivalve has become hot research topic. Manila clam Ruditapes philippinarum is a species marine with important economic value. A new C-type lectin (Rpcl) from was obtained and its potential feeding studied. Rpcl cDNA 929 bp length had 720 open reading frame. encoded 235 amino acids, comprising carbohydrate recognition domain (CRD) as well an...

Carbon isotopic fractionation was studied during the development of diatom Chaetoceros muelleri grown in batch culture with 13C-depleted CO2 addition. Cellular and growth parameters composition dissolved inorganic carbon particulate organic were monitored every two days, while content individual fatty acid polar lipid neutral measured on 5th day (end exponential phase), 10th 14th days (stationa...