Rapid clonal propagation through in vitro techniques using cotyledonary node as an explant has been attempted in the high yielding, tikka-susceptible (ICG 11337, AK 1224, ICGS 44, JL 24) and tikka-immune (ICG 6284) varieties of Arachis hypogaea L. Cotyledonary nodes were excised from the 12–15 days-old axenic seedlings and cultured in the presence of various concentrations of N6 –benzylaminopur...

findings in relation to the inhibitory effects of natural substances against microorganisms showed that herbal products may be alternatives to synthetic and chemical drugs due to their significant therapeutic effects. the pennyroyal ( mentha pulegium ) as an important medicinal plant, belongs to the lamiaceae family is applied in the pharmaceutical and food industries. in recent years, plant ti...

embryogenic calli production in vitis is mainly possible by use of such regenerative tissues as anther and ovary. because of long periods of time required for obtaining such tissues, use of such vegetative organs as leaf explant would be a promising alternative. towards this end, leaf explants were taken from two varieties of ‘flame seedless’ and ‘red sultanina’ to be raised in. ms medium, cons...

In vitro multiple shoot regeneration of Solanum nigrum L., an Indian medicinal plant was accomplished on MS medium utilizing shoot tip and nodal explants. Direct multiple shoots differentiated within 6 weeks when explants were cultured on MS medium containing BAP (1.0-5.0 mg/l) and KIN (1.0-5.0 mg/l) individually. Among various concentrations of cytokinins tested, maximum number of multiple sho...

Objective: In vitro propagation of AZ x M9 new apple rootstock resulted of breeding program of vegetative apple rootstock in Iran was investigated. Methods: Nodal explants were inoculated in Murashige and SKoog (MS) medium supplemented with various concentrations of 6-benzylaminon purine (BAP) (0.4, 0.8 and 1.2 mg/l) alone or with naphthalene acetic acid (NAA) (0.1 mg/l). We examined t...

For the regeneration of shoots, leaf segments of Rehmannia elata were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP) (1, 2, or 4 mg L) with or without 1-naphthalene acetic acid (NAA) used at 0.1 or 0.5 mg L. Regenerated shoots were obtained from the leaf cultures at all BAP concentrations with or without both concentrations of N...

In vitro propagation of AZ x M9 new apple rootstock resulted of breeding program of vegetative apple rootstock in Iran was investigated. Nodal explants were inoculated in Murashige and SKoog (MS) medium supplemented with various concentrations of 6-benzylaminon purine (BAP) (0.4, 0.8 and 1.2 mg/l) alone or with naphthalene acetic acid (NAA) (0.1 mg/l). We examined the effect of differe...

An efficient In vitro protocol was established for callogenesis from various plant parts (Leaf, stem, flower bud and tendril) of Passiflora vitifolia Kunth. On Murashige Skoog (MS) basal medium supplemented with concentration auxins (NAA, IAA, 2,4-D) cytokinins (BAP Kn ). Different concentrations (0.5 to 4 mg/L), IAA 2mg/L), NAA 2 mg/l) cytokinin BAP mg/L) were assessed callus induction. Growth...

background & aim: dracocephalum genus belongs to the lamiaceae family which has eight species in iran. dracocephalum kotschyi boiss is an important medicinal and aromatic plant that use in pharmaceutical industry. in addition, this species is used in iranian traditional medicine. the aim of current study was to examine the various growth regulations and explants on direct organogenesis of d...

The tomato (Solanum lycopersicum L.) is a major crop of global economic significance. characterization genes associated with agriculturally important traits often performed using genetic transformation. To achieve an efficient transformation protocol, three components are required, namely, regenerable target tissue, DNA delivery method, and robust transformant selection system. present study wa...