Objectives: Progressive multifocal leukoencephalopathy (PML) is caused by a reactivated infection with the human polyomavirus JC (JCV). Primary infection with JCV has not been linked to any disease in humans. Using recombinant viral protein 1 (VP1) of JCV as antigen, we developed an enzyme linked immunosorbent assay (ELISA) for the detection of VP1-speciŽc IgM. Methods: Two different direct ELI...

The VP1 transcription factor functions as both a repressor and an activator of gene expression in the developing aleurone. Vp1 activation of the anthocyanin pathway exhibits strict cell autonomy in aleurone. In contrast, Vp1-mediated repression of hydrolase genes in aleurone cells during seed development is determined by a combination of cell autonomous and cell non-autonomous signals. To analy...

The JC polyomavirus virus (JCPyV) affects more than 80% of the human population in their early life stage. It mainly immunocompromised individuals where replication oligodendrocytes and astrocytes may lead to fatal progressive multifocal encephalopathy (PML). Virus protein 1 (VP1) is one major structural proteins viral capsid, responsible for keeping alive gastrointestinal urinary tracts. VP1 o...

Abstract High molecular weight glutenin subunits (HMW GS) represent an important fraction of endosperm-specific seed-storage proteins that provide elasticity to bread dough. Previously, the second cis -regulatory module (CRM2) was found be one most conserved part HMW GS promoters, which indicated its pre-eminent role in their gene regulation. Here, we observed deletion CRM2 from promoters Bx7 a...

Pentamer formation by Vp1, the major capsid protein of simian virus 40, requires an interdigitation of structural elements from the Vp1 monomers [Liddington, R. C., Yan, Y., Moulai, J., Sahli, R., Benjamin, T. L. & Harrison, S. C. (1991) Nature (London) 354, 278-284]. Our analyses reveal that disulfide-linked Vp1 homooligomers are present in the simian virus 40-infected cytoplasm and that they ...

Structural studies have implicated Cys(9), Cys(104) and Cys(207) of simian virus 40 (SV40) Vp1 in disulfide bond formation. Recently, we have shown the three cysteines to be essential for disulfide linkage of Vp1 complexes in vitro. Here, the role of the three cysteines was explored during the course of SV40 infection. Single-, double- and triple-mutant Vp1 at Cys(9), Cys(104) and Cys(207) cont...

In polyomaviruses the pentameric capsomers are interlinked by the long C-terminal arm of the structural protein VP1. The T=7 icosahedral structure of these viruses is possible due to an intriguing adaptability of this linker arm to the different local environments in the capsid. To explore the assembly process, we have compared the structure of two virus-like particles (VLPs) formed, as we foun...

Recent whole-genome analysis has demonstrated limited genetic variation in Chlamydia pneumoniae, with one strain (AR39) containing a 4,524 nucleotide single-stranded DNA bacteriophage, PhiCpn1. Using PCR, reverse transcription (RT)-PCR, and Western blotting, we confirmed the presence and functional expression of PhiCpn1 in C. pneumoniae strain AR39 and its absence in strain CWL029. Six addition...

La enfermedad infecciosa bursal es una patología de origen viral que afecta a aves corral ocasionando alta tasa mortalidad. Las vacunas existentes se enfrentan al problema la aparición nuevas cepas, por lo realizan búsquedas moléculas con potencial afinidad unión proteínas virales. El extracto Withania somnífera interviene en replicación del virus bursitis infecciosa, pero el posible mecanismo ...

Recombinant versions of parvovirus B19 capsid proteins VP1 and VP2 are used for immunodiagnostic assays for detection of antiviral antibodies. The immune response to B19 is characterized by a gradual loss of antibodies directed against linear epitopes of VP2. A similar occurrence for antibodies raised against VP1 protein would represent a limitation to serological assays incorporating denatured...