Objectives: To evaluate the antimicrobial effects of experimental root canal medicaments on Enterococcus faecalis and Candida albicans day 3 7 using quantitative polymerase chain reaction (Q-PCR) analyses. Materials Methods: 240 single-rooted, single-canalled human teeth were used. Root canals shaped mechanically embedded in acrylic blocks, then sterilized an autoclave. The samples divided into...

PURPOSE To evaluate the MMP-inhibitory effect of chlorhexidine when incorporated in the primer of a two-step selfetching adhesive (Clearfil SE Bond). MATERIALS AND METHODS Powdered dentin made from human teeth was treated with Clearfil SE Bond primers containing chlorhexidine of different concentrations for 20 s or for 2 min. The collagenolytic activity of the dentin powder was assayed using ...

Muramyl dipeptide (MDP)-Lys (L18), a synthetic MDP analogue derived from bacterial cell walls, has been reported to be a potent immunoadjuvant that enhances protective immunity against pathogens and tumors by stimulating immune-competent cells, such as monocytes and macrophages. However, it is not known whether MDP-Lys modulates the function of dendritic cells (DCs), which are the most potent a...

Description: The thiolester site of the human alpha-2-macroglobulin (A2M) is essential for the function of A2M as a proteinase inhibitor (1, 2). This site, which is encoded for by exon 24 of the A2M gene, was amplified by polymerase chain reactio (PCRa usinr tho primer 5'-CTGAAGAATTATCCCTGAAAC-3' and 5'-TGGTTCCTGCCATATCGCTC-3'. PCRs were performed in 100 /xl volumes containing 0.5 ng genomic DN...

Penangkapan non-osseous pada pemeriksaan sidik tulang dengan menggunakan radiofarmaka Technetium-99m methylene diphosphonate (Tc-99m MDP) dapat disebabkan karena proses neoplasma, trauma, inflamasi, iskemik, dan artefak. Perubahan metabolisme lesi diketahui pencitraan Tc-99m MDP. Lesi jaringan lunak terlihat adanya peningkatan aktivitas area fokal. Tujuan penulisan telaah pustaka ini adalah unt...

We studied the time course of humoral and cellular immunity of guinea pigs injected with the synthetic adjuvant N-acetylmuramyl L-alanyl-D-isoglutamine (MDP Pasteur, 10 microgram) in Freund incomplete adjuvant; the kinetics were compared with those obtained with Freund complete adjuvant (50 microgram of whole Mycobacterium butyricum). The antibody response to ovalbumin was faster and higher wit...

Development and use of primer sets to amplify nucleic acid sequences of interest is fundamental to studies spanning many life science disciplines. As such, the validation of primer sets is essential. Several computer programs have been created to aid in the initial selection of primer sequences that may or may not require multiple nucleotide combinations (i.e., degeneracies). Conversely, valida...

We have developed a highly sensitive method for DNA analysis on 3D gel element microarrays, a technique we call multiplex microarray-enhanced PCR (MME-PCR). Two amplification strategies are carried out simultaneously in the reaction chamber: on or within gel elements, and in bulk solution over the gel element array. MME-PCR is initiated by multiple complex primers containing gene-specific, forw...