There is considerable evidence that the prion protein binds copper. However, there have also been suggestions that prion protein (PrP) binds manganese. We used isothermal titration calorimetry to identify the manganese binding sites in wild-type mouse PrP. The protein showed two manganese binding sites with affinities that would bind manganese at concentrations of 63 and 200 mum at pH 5.5. This...

Manganese is one of the most abundant metal in natural environments and serves as an essential microelement for all living systems. However, the enrichment of soil with manganese resulting from industrial inputs may threaten terrestrial ecosystems. Several studies have demonstrated harmful effects of manganese exposure by cutaneous contact and/or by soil ingestion to a wide range of soil invert...

Fresh leaves of sorrel (Hibiscus sabdariffa L.) are among the most consumed vegetables in Lubumbashi Democratic Republic Congo (DR Congo). According to literature, this vegetable shows chemical variability depending on culture area. Two varieties species, cultivated and have not yet been studied for their micronutrient content, which fruits main sources. This study investigated mineral total fl...

Very little manganese is imported into Escherichia coli under routine growth conditions: the import system is weakly expressed, the manganese content is low, and a manganese-dependent enzyme is not correctly metallated. Mutants that lack MntH, the importer, grow at wild-type rates, indicating that manganese plays no critical role. However, MntH supports the growth of iron-deficient cells, sugge...

The effect of chemical form and diet on manganese retention was studied in rats using two diets. The corn-skim milk low-manganese diet was fed to three groups of male, weanling albino rats and a purified manganese-free casein-dextrose diet was fed to three other groups. After 7 days on the diets one group on each diet received orally either SaMnCl2, SaMnCl2 plus carrier manganese as MnC12 in th...

BACKGROUND Neurotoxicity and pulmonary dysfunction are well-recognized problems associated with prolonged human exposure to high concentrations of airborne manganese. Surprisingly, histological characterization of pulmonary responses induced by manganese remains incomplete. The primary objective of this study was to characterize histologic changes in the monkey respiratory tract following manga...

There is little information regarding the tissue distribution of manganese in neonates following inhalation. This study determined tissue manganese concentrations in lactating CD rats and their offspring following manganese sulfate (MnSO4) aerosol inhalation. Except for the period of parturition, dams and their offspring were exposed to air or MnSO4 (0.05, 0.5, or 1 mg Mn/m3) for 6 h/day, 7 day...

OBJECTIVE We reviewed the scientific background for the current health-based World Health Organization (WHO) guideline value for manganese in drinking water. DATA SOURCES AND EXTRACTION The initial starting point was the background document for the development of the WHO's guideline value for manganese in drinking water as well as other regulations and recommendations on manganese intake leve...

Apoproteins of cytochrome c peroxidase, horseradish peroxidase, and sperm whale myoglobin were recombined with manganese complexes of proto-, hemato-, meso-, and deuteroporphyrins to form manganese porphyrin-protein complexes. These complexes were purified by column chromatography. All the manganese porphyrin-containing cytochrome c peroxidases were crystallized. Light absorption maxima of mang...