Translation of Hepatitis C Virus (HCV) RNA is directed by an internal ribosome entry site (IRES) in the 5'-untranslated region (5'-UTR). HCV translation is stimulated by the liver-specific microRNA-122 (miR-122) that binds to two binding sites between the stem-loops I and II near the 5'-end of the 5'-UTR. Here, we show that Argonaute (Ago) 2 protein binds to the HCV 5'-UTR in a miR-122-dependen...

Charolais-sired calves from three groups of beef cows, similar in growth potential and mature size but different in genetic potential for milk production (5.6, 7.7, 9.0 kg/d; low, medium, and high, respectively), were allotted to two beef production systems each year for 3 yr. At weaning, calves in an intensive (Int) system went directly into the feedlot for finishing (236 d); calves in an exte...

In comparison with a saline infusion, the infusion of the C-terminal octapeptide of cholecystokinin (4 ng/kg/min) decreased food intake by an average of 122 g in a group of 12 lean men without objective evidence of untoward side effects. Shapes of the cumulative intake curves under the two conditions were similar, but subjects ate less and stopped eating sooner when receiving octapeptide than w...

Deflection profiles of y} and y are shown in Fig. 1 for M -100 kg, 7=490 kN, c = 307 m/sec, a = 226 m/sec, at t = 0.68, 1.02 ane 1.29 sec. At these times the mass positions are * = 52, 118, and 188 m. As shown in Fig. 1, maximum deflection is at the mass location. To determine the adequacy of the simplified mathematical model used in the analyses, the cable deflection at a known position was mo...

The P body protein LSm1 stimulates translation and replication of hepatitis C virus (HCV). As the liverspecific microRNA-122 (miR-122) is required for HCV replication and is associated with P bodies, we investigated whether regulation of HCV by LSm1 involves miR-122. Here, we demonstrate that LSm1 contributes to activation of HCV internal ribosome entry site (IRES)-driven translation by miR-122...

Because our recent studies have demonstrated that miR-122 decreased in whole blood of patients and in whole blood of rats following ischemic stroke, we tested whether elevating blood miR-122 would improve stroke outcomes in rats. Young adult rats were subjected to a temporary middle cerebral artery occlusion (MCAO) or sham operation. A polyethylene glycol-liposome-based transfection system was ...