The influence of parameter changes on the bacterial community of a laboratory-scale anaerobic digester fed with glucose was investigated using a culture-independent approach based on single-strand conformation polymorphism (SSCP) analysis of total 16S rDNA and 16S rRNA amplification products. With the digester operating at steady state, the 16S rDNA SSCP patterns of the bacterial community show...

Here we present the first description of the presence of two distinct types of 16S rRNA genes in the genome of a (eu)bacterium, Thermobispora bispora. We cloned and determined the nucleotide sequences of all four rRNA operons of T. bispora. Sequence comparisons revealed that the genome of T. bispora contains two distinct types of 16S rRNA genes, each type consisting of two identical or nearly i...

The Streptomycetes sp. SCBT strain Gen Bank accession number EU143270 isolated from the rhizosphere soil of medicinal plants was collected from the Kolli hills of Tamil Nadu, India. The strain Streptomyces is designated as School of Chemical and Biotechnology (SCBT), capable of inhibiting the growth of a wide range of Gram-negative and Gram-positive bacteria. An almost complete 16S rRNA gene se...

it is difficult to distinguish between clinically significant slowly-growing, non-pigmented mycobacteria, notably to separate m. aviumand m. intracellulare from one another and from m. scrofulaceum strains. the purpose of this study was to evaluate the extent to which 16s rrna sequencing could be used to highlight the taxonomic relationships of the mycobacterial strains, which are difficult to ...

Discrimination of Staphylococcus aureus strains, isolated from camel abscesses and mastitic milk of camel, cattle and goats, on the basis of 16S-23S ribosomal RNA intergenic space polymorphism was carried out. Two sets of primers were used for amplification of DNA of intergenic space; the one having a highly conserved sequence in eubacterial 23S rRNA transcript, while the other having less cons...

BACKGROUND To take advantage of affordable high-throughput next-generation sequencing technologies to characterize microbial community composition often requires the development of improved methods to overcome technical limitations inherent to the sequencing platforms. Sequencing low sequence diversity libraries such as 16S rRNA amplicons has been problematic on the Illumina MiSeq platform and ...

The human oral microbiome is the most studied human microflora, but 53% of the species have not yet been validly named and 35% remain uncultivated. The uncultivated taxa are known primarily from 16S rRNA sequence information. Sequence information tied solely to obscure isolate or clone numbers, and usually lacking accurate phylogenetic placement, is a major impediment to working with human oral...