Vitellogenic stage carp ovaries were collected aseptically and the cells of ovarian follicles were dispersed by trypsin treatment and cultivated as monolayer. Control cultures were grown in medium M199 alone. Media for experimental cultures were supplemented either with 100 µl ml-1 of CPH and CPS-carp GnRH (cultivated pituitary cell secretion affected by 10-6, 10-8, 10-10 M carp GnRH)....

Steroid estrogens (SEs) are mainly derived from livestock manure, and composting is the common method of bioresource utilization. In this study, an open-air experiment with dairy manure was conducted to observe degradation loss five SEs under influence different precipitation intensities additional mixed fermentation strains. SE determination results for after 30 days indicated that average rem...

Hormones 17α-ethinylestradiol, 17β-estradiol and estriol were analyzed in urban water supply due to the concern about the problem associated with the presence of these compounds in water bodies. The aims of this project were adapt, validate analytical methodology and monitor these hormones in drinking water. Results showed that the limits of detection, linearity, regression coefficients and rec...

This study was to investigate the effect of annexin A5 on testosterone secretion from primary rat Leydig cells and the underlying mechanisms. Isolated rat Leydig cells were treated with annexin A5. Testosterone production was detected by chemiluminescence assay. The protein and mRNA of Steroidogenic acute regulatory (StAR), P450scc, 3β-hydroxysteroid dehydrogenase (3β-HSD), 17β-hydroxysteroid d...