Genomic analysis has revealed heterogeneity among bacterial 16S rRNA gene sequences within a single species; yet the cause(s) remains uncertain. Generalized transducing bacteriophages have recently gained recognition for their abundance as well as their ability to affect lateral gene transfer and to harbor bacterial 16S rRNA gene sequences. Here, we demonstrate the ability of broad-host-range, ...

The 5S ribosomal RNA (rRNA) genes in eukaryotes may occur either interspersed with the other rRNA genes in the ribosomal DNA (rDNA) repeat, or in separate tandem arrays, or dispersed throughout the genome. In Pythium species and in several related Oomycetes, polymerase chain reaction (PCR) amplification of the nontranscribed spacer (NTS) region with one primer specific for the 5S gene revealed,...

Although natural selection appears to favor the elimination of gene redundancy in prokaryotes, multiple copies of each rRNA-encoding gene are common on bacterial chromosomes. Despite this conspicuous deviation from single-copy genes, no phenotype has been consistently associated with rRNA gene copy number. We found that the number of rRNA genes correlates with the rate at which phylogenetically...

The nucleotide sequence of a segment of the mtDNA molecule of Drosophila yakuba which contains the A+T-rich region and the small and large rRNA genes separated by the tRNAval gene has been determined. The 5' end of the small rRNA gene was located by S1 protection analysis. In contrast to mammalian mtDNA, a tRNA gene was not found at the 5' end of the D. yakuba small rRNA gene. The small and lar...

Because of technological limitations, the primer and amplification biases in targeted sequencing of 16S rRNA genes have veiled the true microbial diversity underlying environmental samples. However, the protocol of metagenomic shotgun sequencing provides 16S rRNA gene fragment data with natural immunity against the biases raised during priming and thus the potential of uncovering the true struc...

The DNA coding for ribosomal RNA from Lytechinus variegatus, an Atlantic sea urchin species, is arranged in repeating units. Each unit is cleaved into four fragments by the restriction endonuclease Eco RI. The same gene region is dissected by the enzyme Sal 1 to yield two fragments. The restriction nuclease Kpn I has one restriction site within each repeat unit, thus generating a single fragmen...

The DNA coding for ribosomal RNA from Lytechinus variegatus, an Atlantic sea urchin species, is arranged in repeating units. Each unit is cleaved into four fragments by the restriction endonuclease Eco RI. The same gene region is dissected by the enzyme Sal 1 to yield two fragments. The restriction nuclease Kpn I has one restriction site within each repeat unit, thus generating a single fragmen...

Structure and organization of two complete ribosomal RNA (rRNA) gene sets, rrnO and rrnA, were determined for the first time in Bacillus subtilis. They are located at the region of the replication origin of the chromosome. Each set constitutes a single operon of: two tandem promoters - leader sequence - 16S rRNA gene - Ile-tRNA gene - Ala-tRNA gene - 23S rRNA gene - 5S rRNA gene - termination s...

indole acetic acid is an important hormone that improved the growth of different plant organs such as the root under normal and stress conditions. as various studies have shown, most of rhizobacteria produce indole acetic acid and thereby contribute to the growth of plants. in this study, different rhizobacteria were isolated from different regions of iran for the assessment of their ability to...