Two dimensional polyacrylamide gel electrophoresis (2D-PAGE) maps represent an unavoidable tool in many fields connected with proteome research, such as development of new diagnostic assays or new drugs. Unfortunately the information contained in the maps is often so complex that its recognition and extraction usually requires complex statistical treatments. Statistics accompanies many phases o...

Gel electrophoresis is a powerful experimental method to probe the topology of DNA and other biopolymers. Although there is a large body of experimental work that allows us to accurately separate different topoisomers of a molecule, a full theoretical understanding of these experiments has not yet been achieved. Here we show that the mobility of DNA knots depends crucially and subtly on the phy...

The class of tensor product cubic splines is considered in an optimization process for two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) image alignment problems arising in proteomics studies. Numerical results are presented along with comparisons with previous results based on piecewise affine and bilinear transformations.

In proteomics 2-dimensional SDS-polyacrylamide gel electrophoresis (2D-PAGE) is the most widely used method for analyzing protein mixtures qualitatively. There are, however, a lot of noise and measurement biases which needs to be accounted for both in the localization of spots as well as in the quantitative measurement of protein expression. Previous techniques for denoising 2D gels are based o...

background: in this study, two-dimensional gel electrophoresis (2-de) method was applied to determine and compare the protein spots expressed in the two field isolates of leishmania major and recovered from the patients who were clinically sensitive and resistant to glucantime® treatment. methods: leishmania parasites were isolated from the cutaneous lesions of two cl infected patients in shira...

In recent years, developments in molecular biotechnology have led to the increased promise of detecting and validating biomarkers, or molecular markers that relate to various biological or medical outcomes. Proteomics, the direct study of proteins in biological samples, plays an important role in the biomarker discovery process. These technologies produce complex, high dimensional functional an...

The antigenic specificity of an unusual antinuclear antibody pattern in three patient sera was identified after separating HeLa-cell nuclear extracts by two-dimensional (2D) gel electrophoresis and localizing the antigens by immunoblotting with patient serum. Protein spots were excised from the 2D gel and their contents were analyzed by matrix-assisted laser desorption-ionization (MALDI) or nan...