نتایج جستجو برای: 2neu gene quantification
تعداد نتایج: 1215307 فیلتر نتایج به سال:
اندازه گیری کمیت بار انگل لیشمانیوز یک نشانگر ضروری برای پیشرفت بیماری است هدف از این مطالعه طراحی و اجرای کیت تشخیص کمی بار انگل می باشد پروب و پرایمر از ناحیه its بین ژن های rrna با حداقل تنوع توالی در گونه های شایع لیشمانیوز پوستی طراحی گردید و سپس یک pcr time real به روش taqman probe بصورت کمیت سنجی مطلق (absolut quantification) ایجاد گردید. ژن کلون شده به باکتری escherichia.coli سویه top10...
BACKGROUND Relative quantification is a commonly used method for assessing gene expression, however its accuracy and reliability is dependent upon the choice of an optimal endogenous control gene, and such choice cannot be made a priori. There is limited information available on suitable reference genes to be used for studies involving human epicardial adipose tissue. The objective of the curre...
Gene expression analysis has become an invaluable tool for understanding gene function and regulation. However, global expression analysis requires large RNA quantities or RNA preamplification. We describe an isothermal messenger RNA (mRNA) amplification method, Ribo-SPIA, which generates micrograms of labeled cDNA from 5 ng of total RNA in 1 day for analysis on arrays or by PCR quantification....
Previous studies have suggested that overexpression of HER-2/neu oncogene occurs in 15-40% of breast cancers and that overexpression is associated with poor prognosis. In the present report, we have used an immunohistochemical technique involving a monoclonal antibody specifically reactive with the external domain of HER-2/neu to study expression of HER-2/neu in frozen sections of normal ovary ...
Representative data from KRAS (A) and MET (B) relative gene quantification (RQ) in the three metastatic sites (liver, retroperitoneum and ureteral lesion) using TaqMan Copy Number Assay. The number of copies of the KRAS and MET genes is determined by relative quantification using the comparative CT (??CT) method. MAD1L1 and IL22 genes, respectively on chromosome 7 and 12, were used as reference...
In this paper we provide a simple proof of the fact that for a system of two spin2 particles, and for a choice of a set of observables, there is a unique state which shows Hardy-type nonlocality. Moreover, an explicit expression for the probability that an ensemble of particle pairs prepared in such a state exhibits a Hardy-type nonlocality contradiction is given in terms of two independent par...
the effect of mdma and pentoxifylline drug on bad/bcl-xl gene dosage expression changes on rat liver
mdma generally known as ecstasy, have deleterious effects on the serotonergic neurotransmitter system. recent findings suggest that the liver and brain are major target organs of mdma-related toxicities. although most research is being dynamically performed on brain, however, the molecular mechanisms by which mdma elicits adverse effects in both organs are poorly undrestood.the present study wa...
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