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Several systems have been developed that allow transcription of a target gene to be chemically controlled, usually by an allosteric modulator of transcription factor activity. An alternative is to use chemical inducers of dimerization, or "dimerizers," to reconstitute active transcription factors from inactive fusion proteins. The most widely used system employs the natural product rapamycin, o...
We have previously demonstrated that two distinct transcripts are produced from ORF 50, the major transcriptional activating gene of herpesvirus saimiri. The products of these transcripts trans-activate the delayed-early ORF 6 promoter, though to different degrees. Deletion analysis demonstrated that the ORF 50 responsive elements are contained in a 132 bp fragment situated 127-259 bp from the ...
We report here the crystal structure of an SF3 DNA helicase, Rep40, from adeno-associated virus 2 (AAV2). We show that AAV2 Rep40 is structurally more similar to the AAA(+) class of cellular proteins than to DNA helicases from other superfamilies. The structure delineates the expected Walker A and B motifs, but also reveals an unexpected "arginine finger" that directly implies the requirement o...
If the cosmological constant Λ can not be neglected, we will show in this short report, that graviton should have a rest mass mg = √ 2Λ = √ 2Λh̄c−1 different from zero.
We investigate Room squares and subsquares. All terminology is as in [6] and [7], and we will assume the reader is familiar with the definitions contained therein. (These include group-divisible designs, transverial designs, and frames.) We will refer to an incomplete Room square of side s missing a subsquare of side t as an (s, t)-incompZefe Room square. It is known that for any odd t, there i...
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