The experiment was designed to evaluate the optimal concentration of egg yolk in extender for diluting Barbari buck semen during short-term storage at 4oC. A total of four Barbari bucks were used as semen donors during the experiment. The observed mean (±SE) valuesrecorded for percent live spermatozoa ranged from 63.5 ± 1.08 to 75.33 ± 0.99, percent progressive motile spermatozoa ranged from 54...

Purpose : The aim of this study is to compare the results of two series of penetrating keratoplasties, e.sch with a different method of conservation. Methods : The corneas of sixteen donors were conserved one of each pair at 4OC and the other at 31%. The average duration of conservation was 3.2 days at 4°C and 13.4 days at 31°C. Average endothelial cell density evaluated with trypan blue was 34...

Background: Paroxysmal cold hemoglobinuria (PCH), an autoimmune hemolytic anaemia caused due to polyclonal IgG anti-P autoantibody binding to red blood cell surface antigens and is characterized by hemoglobinuria, typically after exposure to cold temperatures. Case Presentation: An 84-year-old female patient started developing breathing difficulty off and on with generalised weakness and loss o...

The objective of this study was to determine the prevalence of hepatitis B and C among the patients who came for dental treatment to Bacha Khan Medical & Dental College, Mardan. Four hundred patients formed the study group. The blood samples of all these patients were taken in the hospital laboratory and were screened for HBsAg and Anti HCV using immunochromatography (ICT) method. The blood was...

Viral safety is a prerequisite for manufacturing clinical antihemophilic factor VIII concentrates from human plasma. With particular regard to the hepatitis A virus (HAV), a terminal dry-heat treatment (100 degrees for 30 min) process, following lyophilization, was developed to improve the virus safety of a solvent/detergent-treated antihemophilic factor VIII concentrate. The loss of factor VII...

1. The concentrations of the acid soluble and insoluble coenzyme A (CoA) esters were measured in samples of liver obtained at autopsy from Reye’s syndrome and control subjects because the long chain fatty CoA compounds which make up the bulk of the acid insoluble CoA esters are known to inhibit a number of mitochondrially located enzymes, several of which may be affected in Reye’s syndrome. 2. ...

ITTLE attention appears to have been paid in recent years to neurophysioL logical differences between mammals capable of hibernation and those that are not. Horvath (I) pointed out that certain species (ground squirrel, hedgehog, European hamster, and bat) could be cooled artscially until their rectal temperatures approached 0°C. and still revive spontaneously on being returned to a warmer envi...

The direct agglutination test (DAT) based on a freeze-dried antigen and the rK39 dipstick test were evaluated for the sero-diagnosis of visceral leishmaniasis (VL). The sensitivity and specificity of both tests were determined using sera from confirmed VL patients (n = 21), healthy controls (n = 19) and from patients with other confirmed infectious diseases (n = 42). The DAT had a sensitivity a...

To date, very little information exists on recovery methods for freeze-injured cells and the effect of blastor conventional-chilling for reducing specific pathogens associated with pork surfaces. To address these issues, two separate studies were conducted to determine 1) an effective recovery method for freeze-injured Listeria monocytogenes, Salmonella Typhimurium and Campylobacter coli associ...

This experiment was designed to study the effect cooling rate in Hariana bull spermatozoa during cryopreservation. For this purpose, ejaculates were collected from four Hariana bulls using artificial vagina at biweekly interval. The semen sample which possesses more than 70% progressive motility and above 500 million/ml spermatozoa concentration was subsequently subjected to processing for liqu...