BACKGROUND/AIMS This study characterized the safety and pharmacological properties of AVI-005, a novel glycosylated recombinant human interferon-alpha2b produced from the egg whites of chickens transfected with human cDNA. METHODS 18 healthy volunteers received single subcutaneous rising doses (0.5, 1.66 or 5 million international units, MIU) of AVI-005. A randomized parallel comparator group...

A simple procedure for the preparation of plant cell protoplasts is described. The leaf is first treated with pectinase under conditions that do not liberate cells; cellulase is then used to release protoplasts. The yields are comparable to those obtained by methods that entail the prior preparation of free cells. The method is of general application but is particularly useful with tissues that...

Abstract Pectinases, like other industrial enzymes are usually expensive. The use of pineapple peel pectin as substrate is triggered by the large tones waste generated in Nigeria. Oil extraction mechanical/chemical means have associated disadvantages. This research aimed at employing locally produced pectinase for coconut-oil and to compare yield with commercial pectinase. Fifty grammes dried p...

The ‘Crimson seedless’ grape encountered several difficulties during shelf life, including weight loss, rachis browning, and berry shattering. effect of exogenous pectin (PE) polyphenol alcohol (PVA) with supporting salicylic acid (SA) at different concentrations (0, 1, 2 mM) was applied. coating applied to bunches for 5 min stored room temperature (26 ± 1 °C RH 65 3%) 4 days. In this study, po...

Cutinase from Thermobifida fusca is thermally stable and has potential application in the bioscouring of cotton in the textile industry. In the present study, the carbohydrate-binding modules (CBMs) from T. fusca cellulase Cel6A (CBM(Cel6A)) and Cellulomonas fimi cellulase CenA (CBM(CenA)) were fused, separately, to the carboxyl terminus of T. fusca cutinase. Both fusion enzymes, cutinase-CBM(C...

Pectinase (Pectin methyl esterase) produced by Aspergillus niger in solid state fermentation of apple pomace using the optimized conditions was partially purified by (NH4)2SO4 fractionation (20-80% concentration). The purified enzyme was found to be stable up to 60 days. It remained heat stable up to 50oC but completely inactivated at 90oC. The partially purified enzyme showed highest activity ...

BACKGROUND The purpose of the present study is to quantify the in vitro antiproliferative and cytotoxic effects of mitomycin-C on human keratocytes for their potential to modulate corneal stromal wound healing. METHODS Cultured human keratocytes were exposed to various concentrations of mitomycin-C for periods of 5 minutes and 1 hour. Keratocyte proliferation and viability were assessed by ph...

1. The effect of angiotensin 11-amide upon the intrarenal distribution of perfusate was studied in the isolated rat kidney. 2. Low concentrations of angiotensin, 4.86 pmol/l (0-005 pg/l), reduced the flow rate through the papilla but did not alter total flow. 3. Higher concentrations of angiotensin, 0.486 nmol/l(O.5 pg/l), reduced total renal flow but did not decrease papillary perfusion. 4. In...

This manuscript describes the potential of a cutinase from the fungus Fusarium solani pisi for cotton wax degradation in order to design an efficient low temperature scouring process. The main characteristics, relevant to cotton wax removal with F. solani pisi cutinase are given. The additive effect of cutinase on pectinase was investigated and optimum incubation conditions were determined. Com...