−Biodegradation of methylene chloride by a mixed microbial culture, isolated from a common sewage treatment plant, was investigated in a batch system. Batch experiments were performed at room temperature (27 C) and pH value of 7. The methylene chloride concentration in growth media varied from 25 mgl to 250 mgl. A maximum observed degradation was 1 mglh at 100 mgl of methylene chloride. The cul...

Kocaavşar Deresinden Mayıs 2020 ve Ekim tarihlerinde iki istasyondan su örnekleri alınıp suyun bazı fiziksel kimyasal parametreleri ile fitoplanktonik alg florası incelenmiştir. Yapılan analizlerde, sıcaklığı (T) 10-27°C, pH 8.77-14.70, elektriksel kondüktivite (EK) 322-712 μScm-1, nitrat azotu (NO3-N) 1.8-2.5 mgl-1, toplam azot (TN) 2.8-4 fosfat (PO4-P) 0.2-0.34 mgl-1 fosfor (TP) 0.26-0.4 aral...

Abstract Macrophage plasticity and functional activation are critical to the broad array of macrophage-mediated functions that help determine disease outcomes. galactose-type lectin (MGL), a type-2 C-type receptor (CLR) primarily expressed on myeloid cells, has been shown play an anti-inflammatory role in innate response some infectious agents. Our lab recently demonstrated MGL-1 contributes an...

Although sialic acid has long been recognized as the primary receptor determinant for attachment of influenza virus to host cells, the specific receptor molecules that mediate viral entry are not known for any cell type. For the infection of murine macrophages by influenza virus, our earlier study indicated involvement of a C-type lectin, the macrophage mannose receptor (MMR), in this process. ...

The endogenous cannabinoids anandamide (N-arachidonoylethanolamide, AEA) and 2-arachidonoyl glycerol (2-AG) are upregulated during liver fibrogenesis and selectively induce cell death in hepatic stellate cells (HSCs), the major fibrogenic cells in the liver, but not in hepatocytes. In contrast to HSCs, hepatocytes highly express the AEA-degrading enzyme fatty acid amide hydrolase (FAAH) that pr...

An improved understanding of the endocannabinoid system has provided new avenues of drug discovery and development toward the management of pain and other behavioral maladies. Exogenous cannabinoid type 1 (CB1) receptor agonists such as Δ9-tetrahydrocannabinol are increasingly used for their medicinal actions; however, their utility is constrained by concern regarding abuse-related subjective e...

Three genes (mglA, mglB, and mglC) required for active transport of substrate by the methylgalactoside permease were identified in a hybrid ColE1-DNA plasmid isolated from a clone (pLC3-14) of the Clarke-Carbon bank of Escherichia coli genes. A 4.6-kilobase DNA fragment obtained from pLC3-14 was cloned into the plasmid vector pBR322. The presence of the three mgl genes in the resultant plasmid,...

The body maintains Mg(2+) homeostasis by renal and intestinal (re)absorption. However, the molecular mechanisms that mediate transepithelial Mg(2+) transport are largely unknown. Transient receptor potential melastatin 6 (TRPM6) was recently identified and shown to function in active epithelial Mg(2+) transport in intestine and kidney. To define the relationship between Mg(2+) status and TRPM6 ...

Gonorrhea is one of the most prevalent sexually transmitted diseases in the world. A naturally occurring variation of the terminal carbohydrates on the lipooligosaccharide (LOS) molecule correlates with altered disease states. Here, we investigated the interaction of different stable gonoccocal LOS phenotypes with human dendritic cells and demonstrate that each variant targets a different set o...