Polymorphisms in mitochondrial DNA (mtDNA) were analyzed in 152 samples from the Polish population using restriction enzymes AvaI, BamHI, HaeII, HpaI and PstI. Additionally, each sample was classified into the appropriate haplogroup. When required, appropriate fragments were sequenced to establish the exact polymorphic sites. We found one new morph for PstI and six new morphs for AvaII. Some de...

Source and Description: G2—2 E is a single copy 200 bp subfragment of G2 cloned into the EcoRV site of Bluescript KSII(+). Clone G2 was isolated from a library highly enriched for (TTAGGG)n-associated sequences (1). Polymorphisms: TaqI identifies a two allele polymorphism as assessed in 29 unrelated Caucasians. Allele Size No. of Chromosomes Frequency Al 900 bp 31 (0.54) A2 700 bp 27 (0.46) Pst...

Background: Pulmonary tuberculosis (PTB) has recently become a major problem in developed countries especially in immune compromised HIV infected individuals. Cytokines, their genes and receptors have been implicated in the protective immunity, pathophysiology and development of tuberculosis.   Material & Methods: In the present study the genotype frequencies of a number of polymorphic genes co...

Polymorphism of the second exon of the caprine leukocyte antigen-DRB3 gene (CLA-DRB3*02) was investigated in this study. The 285 bp PCR product of 258 individuals from 10 domestic goat breeds in Southwest China was digested with restriction endonucleases PstI and HaeIII and then genotyped. Three alleles and 4 restriction digestion profiles were distinguished by digestion of the PCR fragment by ...

background: pulmonary tuberculosis (ptb) has recently become a major problem in developed countries especially in immune compromised hiv infected individuals. cytokines, their genes and receptors have been implicated in the protective immunity, pathophysiology and development of tuberculosis.   material & methods: in the present study the genotype frequencies of a number of polymorphic genes co...

There is a possible association between acute leukemia (AL) and polymorphisms in genes coding for cytochrome P4502E1 (CYP2E1) and myeloperoxidase (MPO), the enzymes both involved in the metabolism and bioactivation of xenobiotic compounds, including benzene especially. Previous studies reported conflicting results. Therefore, a meta-analysis of available molecular epidemiologic studies was perf...