Acanthamoeba keratitis has been increasing in recent years. Main risk factors are contact lens wear and their cleaning solutions. Most contact lens wearers use multipurpose disinfecting solutions (MPDS) for cleansing and disinfecting microorganisms because of its convenience. We determined amoebicidal effects of MPDS made in Korea and their cytotoxicity on human corneal epithelium cells. Fiftee...

Acanthamoeba castellanii mannose-binding protein (MBP) mediates adhesion of the amoebae to corneal epithelial cells, a key first step in the pathogenesis of Acanthamoeba keratitis (AK), a devastating corneal infection. In the present study, we demonstrate that oral immunization with recombinant MBP ameliorates AK in a hamster animal model and that this protection is associated with the presence...

Abstract Free-living amoebae (FLA) are protozoa dispersed in different environments and responsible for infections caused to humans other animals. Microorganisms such as Acanthamoeba spp., Vermamoeba sp., Naegleria sp. associated with diseases that affect the central nervous system, addition skin keratitis, occurs genus vermiformis. Due concerns of these FLA anthropogenic aquatic environments, ...

purpose: to assess the efficacy of polyhexamethylene biguanide (phmb) in treating acanthamoeba keratitis (ak) and also to identify related factors affecting final visual outcome in ak patients treated with phmb methods: in these interventional case series study, 27 eyes of 25 patients with ak received phmb 0.02% and divided into two groups according to the final visual acuity (va) va equal to o...

OBJECTIVES Microbial keratitis accounts for up to 30% of blindness in some less developed societies. The development of a single broad-spectrum topical antimicrobial effective against bacteria, fungi and Acanthamoeba would have a major impact on reducing the morbidity and simplifying the treatment of microbial keratitis. To this end, the activity of the amidoamine myristamidopropyl dimethylamin...

Antifungal drugs have been proposed as a novel treatment for Acanthamoeba keratitis. The cysticidal activity of several antifungal compounds was tested against different genotypes of culture collection and clinical isolates of Acanthamoeba. Only voriconazole and posaconazole were found to be cysticidal, with no differences in activity observed between clinical and culture collection isolates.

Acanthamoeba is a free-living ameba that is found throughout the world and that causes encephalitis, keratitis, and cutaneous infections in humans. It has two stages in its life cycle: a trophic stage and a resistant cyst stage. We describe here the ability of Acanthamoeba cysts to survive desiccation for more than 20 years.