In vivo synthesis of cellulose by Acetobacter xylinum was monitored by darkfield light microscopy. Cellulose is synthesized in the form of a ribbon projecting from the pole of the bacterial rod. The ribbon elongates at a rate of 2 mum min-1. The ribbon consists of approximately 46 microfibrils which average 1.6 X 5.8 nm in cross section. The observed microfibrillar elongation rate corresponds t...

In this study, the removal of mercury ions by cellulose of Acetobacter xylinum was investigated in the synthetic and chlor-alkali wastewater. Biofilms of Acetobacter xylinum were grown in laboratory column bioreactors. The biofilms were continuously treated with sterile synthetic model wastewater or nonsterile, neutralized chloralkali wastewater.The extent of adsorption was studied as function ...

Gut microorganisms are essential for the nutritional health of many animals, but the underlying mechanisms are poorly understood. This study investigated how lipid accumulation by adult Drosophila melanogaster is reduced in flies associated with the bacterium Acetobacter tropicalis which displays oral-faecal cycling between the gut and food. We demonstrate that the lower lipid content of A. tro...

Abstract Acetic acid bacteria (AAB) can selectively oxidize diols into their corresponding hydroxyacids. Notably, they convert 1,3-propanediol (1,3-PDO) 3-hydroxypropionic (3-HP), which is a promising building-block. Until now, 3-HP production with AAB carried out in batch and using resting cells at high cell densities (up to 10 g L −1 of dry weight). This approach likely limited by detrimental...

(a) Sorbose (L-sorbose, sorbinose) (C6H12O6, CAS Reg. No. 87–79–6) is an orthorhombic, bisphenoidal crystalline ketohexose. It was originally identifed in the juice of mature berries from the mountain ash (Sorbus aucuparia) where it occurs as the result of microbial oxidation of sorbitol. It also occurs naturally in other plants. Sorbose can be synthesized by the catalytic hydrogenation of gluc...

The family Acetobacteraceae currently includes three known nitrogen-fixing species, Gluconacetobacter diazotrophicus, G. johannae and G. azotocaptans. In the present study, acetic acid-producing nitrogen-fixing bacteria were isolated from four different wetland rice varieties cultivated in the state of Tamilnadu, India. Most of these isolates were identified as G. diazotrophicus on the basis of...

Animals host multi-species microbial communities (microbiomes) whose properties may result from inter-species interactions; however, current understanding of host-microbiome interactions derives mostly from studies in which elucidation of microbe-microbe interactions is difficult. In exploring how Drosophila melanogaster acquires its microbiome, we found that a microbial community influences Dr...

Cellulose production from sucrose by Acetobacter strains is accompanied by the accumulation of a water-soluble polysaccharide, called levan. To improve cellulose productivity, a levansucrase-deficient mutant, LD-2, was derived from Acetobacter strain 757 and used as a host for the construction of recombinant strains. An LD-2 mutant harboring a plasmid containing the sucrase gene, sucZE3, from Z...