Galactosyltransferaseactivity (UDP-galactose:A/-acetyl-o-glucosamine-D-galactosyltransferase) couldbemeasuredinthymus and sera from differentstrainsof mice.Total thymic homogenatesor thymocytepreparationsobtainedfromthymomacarry ing AKR/J miceexhibitedhigherenzymeactivity comparedto nonleukemic ontrolmice.A similardifferencewasalsonotedin Swiss mousethymus which developthymic leukemiaupon a sin...

Galactosyltransferaseactivity (UDP-galactose:A/-acetyl-o-glucosamine-D-galactosyltransferase) couldbemeasuredinthymus and sera from differentstrainsof mice.Total thymic homogenatesor thymocytepreparationsobtainedfromthymomacarry ing AKR/J miceexhibitedhigherenzymeactivity comparedto nonleukemic ontrolmice.A similardifferencewasalsonotedin Swiss mousethymus which developthymic leukemiaupon a sin...

In order to explore how molecules are linked to the membrane surface in larval Taenia solium, whole cysticerci were incubated in the presence of phospholipase C from Clostridium perfringens (PLC). Released material was collected and analyzed in polyacrylamide gels with sodium dodecyl sulfate. Two major bands with apparent molecular weights of 180 and 43 kDa were observed. Western blot of releas...

The extracts from 30 species of the green algae were examined for hemagglutination activity with a variety different animal and human erythrocytes which untreated treated enzymes. Almost all showed at least toward one type tested. Strong was detected in 5 Chlorophyta (Anadyomene plicata, Avrainvillea erecta, Boodlea struveoides, Halimeda velasquezii discoidea) enzyme-treated rabbit sheep erythr...

Hemagglutination and intestinal adherence properties of non-O1 Vibrio cholerae were studied in vitro. No definite correlation between the cell-associated hemagglutinin titers and the intestinal adhesion indices was noted. Sugar- and glycoprotein-mediated inhibition data also indicated differences between the hemagglutination and adherence processes in respect to the receptor structures. Intesti...

Porous membranes with glycopolymer brushes were prepared as biomaterials for affinity separation. Glycopolymer brushes contained acrylic acid and D-mannose or N-acetyl-D-glucosamine, and were formed on substrates by surface-initiated atom transfer radical polymerization. The presence of glycopolymer brush was confirmed by X-ray photoelectron spectroscopy, contact angle, and ellipsometry measure...

Phosphoglucomutase 3 (PGM3) is an enzyme converting N-acetyl-glucosamine-6-phosphate to N-acetyl-glucosamine-1-phosphate, a precursor important for glycosylation. Mutations in the PGM3 gene have recently been identified as the cause of novel primary immunodeficiency with a hyper-IgE like syndrome. Here we report the occurrence of a homozygous mutation in the PGM3 gene in a family with immunodef...

The production of extracellular poly-β-1,6-N-acetyl-D-glucosamine (PNAG) by Staphylococcus epidermidis is the principal determinant of biofilm formation on indwelling medical devices. Enzymes that degrade PNAG therefore provide an attractive strategy for biofilm removal and for the manufacture of biofilm-resistant coatings. Here we present methods that allow the identification of PNAG-degrading...

Zeleznick, L. D. (CIBA Pharmaceutical Co., Summit, N.J.), H. Hankin, J. J. Boltralik, H. Heymann, and S. S. Barkulis. Purification and properties of N-acetyl-d-glucosamine kinase from Streptococcus pyogenes. J. Bacteriol. 88:1288-1295. 1964.-A kinase from Streptococcus pyogenes which catalyzes adenosine triphosphate-dependent phosphorylation of d-glucose and N-acetyl-d-glucosamine has been puri...

In a previous study on heparin biosynthesis, incubation of a mouse mastocytoma microsomal fraction with UDP-N-acetyl-D-glucosamine and UDP-D-glucuronic acid resulted in the formation of two nonsulfated polysaccharides, one of which was fully N-acetylated whereas the other contained about equal amounts of Nacetylated and N-unsubstituted glucosamine residues. In the presence of 3’-phosphoadenylyl...