For many years, Taq polymerase has served as the stalwart enzyme in the PCR amplification of DNA. However, a major limitation of Taq is its inability to amplify damaged DNA, thereby restricting its usefulness in forensic applications. In contrast, Y-family DNA polymerases, such as Dpo4 from Sulfolobus solfataricus, can traverse a wide variety of DNA lesions. Here, we report the identification a...

The study and development of new sustainable mining methods to exploit low-grade ores secondary metallic resources are essential meet global demand contribute caring for the environment. Copper is one most widely used metals chalcocite main sulfide this metal. Therefore, copper recovery from chalcocite-dominant minerals could have a great impact on industry. In study, we assess at bench scale f...

A 3382 bp fragment containing a gene for a DNA ligase from the extremely thermophilic, acidophilic, and facultatively anaerobic archaeon (archaebacterium) Desulfurolobus ambivalens was cloned and sequenced. The deduced amino acid sequence (600 amino acids, 67619 molecular weight) showed 30-34% sequence identity with the ATP-dependent eucaryal (eukaryotic) DNA ligases of Schizosaccharomyces pomb...

Sverige beskrivs ibland som världens mest sekulariserade land, men det saknas forsk­ning om de trosföreställningar eleverna faktiskt bär med sig in i religionskun­skapsundervisningen. Syftet denna artikel är att undersöka ambivalens kring över­naturliga föreställningar hos unga svenskar, och inleda ett samtal hur religions­kunskapslärare kan överbrygga klyftan mellan elever identifierar religiö...