The telomeric repeat amplification protocol (TRAP) assay has been used to test telomerase activity in numerous cancer specimens. We describe primers, controls and quantification methods for the TRAP assay to accurately measure the level of telomerase activity in clinical samples. The assay is reliable and reproducible in routine analyses and can be used to estimate the processivity of telomeras...

The notation and terminology used here are introduced in the following papers: [13], [14], [3], [9], [2], [7], [1], [4], [5], [10], [6], [12], [11], and [15]. The following proposition is true (1) For all real numbers x, y such that 0 ≤ x < y there exists a real number s0 such that 0 < s0 and x < y 1+s0 < y. The scheme RecExD3 deals with a non empty set A, an element B of A, an element C of A, ...

Linguistic quantifiers are quantifiers defined by linguistic expressions like “around 5” or “most of,” and many types of linguistic quantifiers can be found in the literature (Diaz-Hermida, Bugarin, & Barro, 2003; Glockner, 1997, 2004a, 2004b; Losada, Díaz-Hermida, & Bugarín, 2006) (as semifuzzy quantifiers which allow modeling expressions like “there are twice as many men as women”). We limit ...

BACKGROUND Nuclear medicine imaging of neuroendocrine tumours is performed either by SPECT/CT imaging, using 111In-octreotide or by PET/CT imaging using 68Ga-radiolabelled somatostatin analogs. These imaging techniques will give different image quality and different detection thresholds for tumours, depending on size and activity uptake. The aim was to evaluate the image quality for 111In-SPECT...

Objective: Measurement of blood-brain permeability dysfunction in active and chronic MS lesions with T1-weighted dynamic contrast-enhanced MRI to show variation in inflammatory activity Background: blood-brain-barrier perfusion characterization impaired in MS as some studies have shown recently buta comparison between perfusion parameters in contrast-enhanced and non-enhanced lesions not have ...

Highlights • Reconstruction of mitochondrial protein import and cofactor metabolism in the model eukaryote Saccharomyces cerevisiae Quantification proton motive force shows significant energy cost metabolite transport Protein activity depends on growth rate respiratory iron-sulfur cluster requirements show dependence

We define the notion of “potential existence” by starting from the fact that in multi-valued logic the existential quantifier is interpreted by the least upper bound operator. Besides, we try to define in a general way how to pass from potential into actual

In the presence of suitable power spaces, compactness ofX can be characterized as the singleton {∅} being open in the space A(X). Equivalently, this means that universal quantification over a compact space preserves open predicates. Using the language of represented spaces, one can make sense of notions such as a Σ 2 subset of the space of Σ 2 -subsets of a given space. This suggests higher-ord...

An easy and fast gel diffusion assay for detecting and monitoring lipase activity by quantification of fluorescein is described. By measuring the intensity of fluorescein, it is possible to obtain a calibration curve with a regression coefficient better than by using the radius of fluorescent haloes. Through the quantification of fluorescence intensity of fluorescein released after the hydrolys...

Biofilm cells typically exhibit increased resistance to antimicrobial agents and the host immune system. In vitro antibiofilm activity has been studied widely in the present’s days. It has become necessary to apply various microscopic techniques in order to evaluate in situ anti-biofilm activity and ultrastructural changes. A wide variety of methods can be applied for visualization and quantifi...