A centrifugal force-based adhesion assay has been used to quantitatively examine the kinetics of formation of cell-cell contacts mediated specifically by expression of E-cadherin under the control of a glucocorticoid-inducible promoter in mouse fibroblasts. Analysis of cells expressing maximal or minimal levels of E-cadherin showed that the strength of E-cadherin-mediated adhesion developed in ...

Adhesion GPCRs are structurally identified on the basis of a large extracellular region, similar to Class B GPCR, but which is linked 7TM region by GPCR autoproteolysis-inducing (GAIN) domain [10] containing proteolysis site (GPS). The N-terminal often shares structural homology with adhesive domains (e.g. cadherins, immunolobulin, lectins) facilitating inter- and matricellular interactions lea...

background : vaginal epithelium is the most important site for initial contact of the parasite infection in humans.   objective: to investigate the effect of lactobacillus acidophilus (la) and ph on attachment of t. vaginalis isolated from symptomatic and asymptomatic patients to vaginal epithelial cells (vecs).   methods: following 1-4 hrs contact of parasite with vecs, wet mounts were prepare...

It is now becoming clear that additional transmembrane components can modify integrin-mediated adhesion. Syndecan-4 is a transmembrane heparan sulfate proteoglycan whose external glycosaminoglycan chains can bind extracellular matrix ligands and whose core protein cytoplasmic domain can signal during adhesion. Two papers in this issue of JCS demonstrate, through transfection studies, that synde...

The β1, β2, and β4 subunits of voltage-gated sodium channels reportedly function as cell adhesion molecules. The present crystallographic analysis of the β4 extracellular domain revealed an antiparallel arrangement of the β4 molecules in the crystal lattice. The interface between the two antiparallel β4 molecules is asymmetric, and results in a multimeric assembly. Structure-based mutagenesis a...

BACKGROUND Deleted in liver cancer 1 (DLC1) is a Rho GTPase-activating protein (RhoGAP) frequently deleted and underexpressed in hepatocellular carcinoma (HCC) as well as in other cancers. Recent independent studies have shown interaction of DLC1 with members of the tensin focal adhesion protein family in a Src Homology 2 (SH2) domain-dependent mechanism. DLC1 and tensins interact and co-locali...

The CD58 binding site on human CD2 was recently shown by nuclear magnetic resonance structural data in conjunction with site-directed mutagenesis to be a highly charged surface area covering approximately 770A2 on the major AGFCC'C" face of the CD2 immunoglobulin-like (Ig-like) NH2-terminal domain. Here we have identified the other binding surface of the CD2-CD58 adhesion pair by mutating charg...

in the development of neuroinflammatory diseases, alterations of the blood brain barrier (bbb) represent key events. the integrity of the bbb is partially maintained by endothelia cells (ecs), since they actively limit the transmigration of immune cells. however, the factors that cause endothelial cells to develop an immune cell-permissive phenotype are poorly understood. in general, it has bee...

background: when provisional restorations are worn for long term period, the adhesion of bacteria becomes a primary factor in the development of periodontal diseases. the aims of this study were to evaluate the surface roughness and bacterial adhesion of four different provisional fixed prosthodontic materials. methods: ten cylindrical specimens were prepared from bis-acrylic composites (previs...