We have previously shown that coculture of human embryonic stem cells (hESCs) for 14 days with immortalized fetal hepatocytes yields CD34 cells that can be expanded in serum-free liquid culture into large numbers of megaloblastic nucleated erythroblasts resembling yolk sac– derived cells. We show here that these primitive erythroblasts undergo a switch in hemoglobin (Hb) composition during late...

INTRODUCTION Thalassemia and iron deficiency may both result in hypochromic microcytic anemia. Hematological algorithms that differentiate the two are mainly established in adult selected diagnostic groups. We aimed at creating an algorithm applicable in the presence of children, hemoglobin variants, and iron deficiency. METHODS Our study material constituted blood samples referred during 1 y...

The adult Japanese monkey (macaca fuscata fuscara) hemoglobin was converted into globin by removal of heme. The globin was separated into ƒ¿ and,ƒÀ • @ polypeptide • @ chains • @by • @ countercurrent • @ distribution • @ with • @the • @ solvent system of trichloroacetic acid containing sec-butanol, propionic acid, and water. • @ The • @ ƒ¿ • @ polypeptide • @ chain • @ was • @ hydrolyzed • @ wi...

We have previously shown that coculture of human embryonic stem cells (hESCs) for 14 days with immortalized fetal hepatocytes yields CD34(+) cells that can be expanded in serum-free liquid culture into large numbers of megaloblastic nucleated erythroblasts resembling yolk sac-derived cells. We show here that these primitive erythroblasts undergo a switch in hemoglobin (Hb) composition during la...

Increasing fetal hemoglobin (HbF) levels in adult humans remains an active area in hematologic research. Here we explored erythroid-specific LIN28A expression for its effect in regulating gamma-globin gene expression and HbF levels in cultured adult erythroblasts. For this purpose, lentiviral transduction vectors were produced with LIN28A expression driven by erythroid-specific gene promoter re...

Hemoglobin is the iron-containing oxygen-transport metalloproteins in the red cells of the blood in mammals and other animals. A spheroidal heme protein having four subunits each consisting of a globular protein non-covalently bound, with an embedded heme group. Hb has a molecular weight of about 64456. The globular protein units of Hb is made up of two identical pairs of polypeptide chains, i....

We have attempted to identify some novel genes which were found to be more highly expressed in the embryonic brain than in the adult brain. Consequently, one of these clones was identified as alpha-globin cDNA. Actually, alpha-globin mRNA was detected in the neurons. In addition, beta-globin mRNA was detected in the neurons as well. Both globin mRNAs were developmentally regulated in the same p...

T HE NATURE of the transition from hemoglobin F synthesis to hemoglobin A synthesis in the fetus and newborn remains unknown. Although it is evident from the elution method developed by Kleihauer, Braun and Betke13 that hemoglobin F and hemoglobin A may coexist in single erythrocytes,24 quantitative data regarding the amounts of these hemoglobins in individual cells are lacking. An attempt to q...

T HE NATURE of the transition from hemoglobin F synthesis to hemoglobin A synthesis in the fetus and newborn remains unknown. Although it is evident from the elution method developed by Kleihauer, Braun and Betke13 that hemoglobin F and hemoglobin A may coexist in single erythrocytes,24 quantitative data regarding the amounts of these hemoglobins in individual cells are lacking. An attempt to q...