نتایج جستجو برای: affinity purification
تعداد نتایج: 136850 فیلتر نتایج به سال:
Recently, "superbinder" SH2 domain variants with three amino acid substitutions (sSH2) were reported to have 100-fold or greater affinity for protein-phosphotyrosine (pY) than natural SH2 domains. Here we report a protocol in which His-tagged Src sSH2 efficiently captures pY-peptides from protease-digested HeLa cell total protein extracts. Affinity purification of pY-peptides by this method sho...
Fusion protein systems can facilitate the purification of proteins produced by recombinant DNA technology (1-3). In these systems, a tag is fused to a target protein. The affinity of the tag for a specific ligand is exploited for the purification of the fusion protein. Here, we demonstrate that during affinity purification many truncated polypeptides carrying the affinity tag are copurified wit...
Artifactual immunofluorescent labelling in yeast, demonstrated by affinity purification of antibody.
In the course of making antibodies against various yeast (S. cerevisiae) proteins, we have noted that it is common to observe reactivity of rabbit sera with a number of extraneous bands on Western transfers of yeast proteins. The pattern of reactive bands can change within a period of weeks, even when the rabbit has not been injected with antigen. A simple method of affinity purification, using...
Kinetic capillary electrophoresis (KCE) is defined as capillary electrophoresis of species that interact during electrophoresis. KCE can serve as a conceptual platform for development of homogeneous kinetic affinity methods for affinity measurements (measurements of binding parameters and quantitative measurements) and affinity purification (purification of known molecules and search of unknown...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید