Material and Methods: The present study was conducted on 40 specimens of breast carcinoma out of which 12 cases were of Grade II and 28 cases were of Grade III. Percentage positivity and scoring of Ki-67 and mean AgNOR count was done with relation to grades. Observations: Ki-67 positivity was seen in 12 cases with the percentage positivity of 2.33±5.516 in grade II and 9.67±7.671 in grade III t...

Argyrophilic staining of nucleolar organizer regions (AgNOR) has been considered to be useful in the diagnosis and prognostic evaluation of different cutaneous tumors. In order to evaluate the role of the AgNOR technique in discriminating aggressive from non-aggressive basal cell carcinoma (BCC), paraffin-embedded histologic sections from 30 cases of aggressive BCC (BCC2) and 30 cases of n...

  Background and Objectives: Uterine smooth muscle tumors are the most common human neoplasm .They are divided clinically as benign and malignant but there is  another group of  lesions which is difficult to place in these two categories ,so-called smooth  muscle tumors of uncertain malignant potential (STUMP) and differentiation  of these tumors on the basis of H&E staining is impossible . Th...

In order to assess the proliferative behaviour of ameloblastoma epithelium nine cases this neoplasm were retrieved from files Oral Pathology Service laboratory Rio Grande do Sul Federal University School Dentistry. Two proliferation marker techniques employed: AgNor - argyrophilic nucleolar organizer regions staining and cell nuclear antigen (PCNA) immunohistochemical assessment technique. Each...

BACKGROUND Apart from a number of cases of inaccurate prognosis in regard to individual patients, the inter- and intra-observer variability of the classical, histological prognosis parameters have been under repeated discussion. For this reason, a long-term analysis was carried out in regard to overall survival by means of automated microscopic image analysis of the nucleolar organizer regions ...

Objective-To investigate whether and to what extent the two major AgNOR proteins, nucleolin and protein B23, are maintained after one cell division in proliferating cells.Design-Using three asynchronously growing human cancer cell lines, TG, SJNKP, and CHP 212 cells, nucleolin and protein B23 were first identified on SDS-polyacrylamide separated nucleolar proteins, transferred to nitrocellulose...

Background: Cell proliferation is an important factor in the prognosis of malignant neoplasia. The number of argyrophilic nucleolar organizer regions (AgNORs) per cell has been considered as an indicator of the cellular proliferative activity. A study is carried out to examine whether AgNOR numbers relate to the growth rate in squamous cell carcinoma (SCC) of the oral cavity. Aim: To verify the...

AIM To evaluate the interphase ribosomal RNA cistron activity of cardiomyocytes in surgical patients with chronic ischaemic heart disease by means of the nucleolar organiser region silver staining (AgNOR) technique. METHODS Nucleoli were investigated in myocardial samples obtained from 46 patients with chronic ischaemic heart disease before, during, and soon after cardioplegia ischaemia. Cryo...