AIM To evaluate the proliferative potential and the cell proliferation rate of odontogenic epithelial cells. MATERIALS AND METHODS Forty-two cases of pericoronal follicles of impacted third molars were submitted to silver impregnation technique for quantification of argyrophilic nucleolar organizer regions (AgNOR) and immunohistochemical staining for EGFR and Ki-67. For AgNOR quantification, ...

AIMS A sound predictive test is lacking for the identification of cirrhotic patients at high risk of developing hepatocellular carcinoma. The present study evaluates the measurement of hepatocyte expression of silver stained nucleolar organiser region (AgNOR) proteins as a risk factor for the development of hepatocellular carcinoma in cirrhosis. METHODS Liver biopsies from 176 cirrhotic patie...

The diagnosis of uterine smooth muscle neoplasms by light microscopy is difficult. Multiple classification schemes have been proposed based on mitotic rate, nuclear atypia, and the presence or absence of necrosis. None of these classification systems has been entirely successful. This study was undertaken to evaluate the use of selected immunohistochemical and histochemical markers in different...

AIM To evaluate the haemopoietic function of bone marrow blood forming cells in human myelodysplastic syndromes (MDS) by silver staining of nucleolar organiser regions (AgNORs). METHODS Nucleoli were investigated in bone marrow blast cells and in erythroid, granulocytic, and megakaryocytic cells from 12 haematologically healthy subjects, and from 26 patients with MDS, including 14 with refrac...

Aims-To define the relation between the quantity of silver stained nucleolar organiser regions (AgNORs) and histological grade, clinical stage, DNA content, and MIB-1 immunostaining in needle biopsy specimens of prostatic adenocarcinomas.Methods-Histological grade was determined according to the Gleason system. AgNOR quantity, DNA content and MIB-1 immunostaining were evaluated by image cytomet...

BACKGROUND The present study is aimed to assess the usefulness of silver nitrate staining of nucleolar organizer regions (NORs) as a quantitative criterion for the diagnosis of selected head and neck tumors. MATERIALS AND METHODS The silver nitrate staining technique was used on 195 paraffin blocks collected from 85 patients. The samples consisted of 21 squamous cell carcinoma (SCC) of larynx...

Nucleolar organizer regions (NORs) are loops of DNA that transcribe to ribosomal RNA. They are associated with nonhistone nucleoproteins which can be stained with silver (AgNORs). The present retrospective study was done to study the efficacy of AgNOR technique in differentiating between non neoplastic and neoplastic lesions of endometrium and to assess the relationship between AgNOR counts and...

Purpose: To verify the relationship between AgNOR expression and lung tissues changes of Wistar rats after pulmonary instillation of benzo[a]pyrene (B[a]P). Methods: Male Rattus norvegicus albinus, Wistar lineage were given a single intrapulmonary instillation of B[a]P at doses of 10 and 20 mg/kg in a volume of approximately 0,3 ml. After 7 and 21 days the rats were killed and the lung slices s...

With 82 species currently described, the genus <i>Leptodactylus</i> is most diverse and representative one in family Leptodactylidae. Concerning chromosomal organization, this represents an interesting underexplored group since data from molecular cytogenetics are incipient, little known about organization distribution of repetitive DNA elements karyotypes. In sense, study aimed at ...

The quantitative distribution of AgNOR proteins represents a reliable index for predicting the clinical outcome of neoplastic patients. The rationale for the utilization of the AgNOR parameter in tumor pathology is based on the following observations: (1) the total quantity of AgNOR proteins evaluated in situ in human cancer cell lines by morphometric analysis is related to the rapidity of cell...