Eggshell membrane (ESM) is considered food waste or a by-product of the egg processing industry. It has huge application potential in engineering, nutraceutical, pharmaceutical, and cosmeceutical sectors. Recently, researchers have shown an increased interest ESM hydrolysate (ESMH) from enzymatic hydrolysis biomaterial applications. Nonetheless, there lacking information on characteristics ESMH...

Please cite this article in press as: Latif, S., & j.foodchem.2010.09.064 In the present work we evaluated five enzyme-mixtures (Protex 7L, Alcalase 2.4L, Viscozyme L, Natuzyme, and Kemzyme) for their effectiveness in extracting the oil and protein recovery from sesame seeds during an enzyme-assisted aqueous extraction (EAAE) process. Alcalase 2.4L was found to be the best for attaining a high ...

The retail meat industry produces a significant amount of waste, containing proteins, lipids, and other elements that could serve as the basis for products. This work presents results research on enzymatic hydrolysis waste green technology to obtain products with added value substitute raw materials. pH, temperature, protease/proteinic substrate ratio (Eo/So), lipolase/lipidic (Eo’/So’) were st...

Influence of amino acid compositions and peptide profiles on antioxidant capacities of two protein hydrolysates from skipjack tuna (Katsuwonus pelamis) dark muscle was investigated. Dark muscles from skipjack tuna were hydrolyzed using five separate proteases, including pepsin, trypsin, Neutrase, papain and Alcalase. Two hydrolysates, ATH and NTH, prepared using Alcalase and Neutrase, respectiv...

Enzymatic hydrolysis has been successfully used for the extraction of numerous biologically active components from a wide variety of natural sources. In the present study, velvet antler was subjected to the extraction process using Alcalase protease. We analyzed bioactive components, such as uronic acid, sulfated-glycosaminoglycans (sulfated-GAGs), and sialic acid, present in the velvet antler ...

Antioxidative and antihypertensive bioactive peptides were successfully derived from Parkia speciosa seed using alcalase. The effects of temperature (25 and 50 °C), substrate-to-enzyme ratio (S/E ratio, 20 and 50), and incubation time (0.5, 1, 2 and 5h) were evaluated based on 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and angiotensin-converting enzyme (ACE) ...

An integrated approach combining liquid chromatography/tandem mass spectrometry (LC– MS/MS) with in silico analysis was used to screen dipeptidyl peptidase IV (DPP-IV) inhibitory peptides from deer skin hydrolysates. A total of 203, 244 and 60 peptides were identified from deer skin hydrolysates prepared with pepsin, pepsin + trypsin and pepsin + Alcalase, respectively, by LC–MS/MS. The percent...

ABSTRACT: The effect of methods to remove protein content on the properties glucosamine hydrochloride from shells white leg shrimp (Litopenaeus vannamei) and black tiger (Penaeus monodon) was investigated. Chitin obtained by demineralization in 6% HCl for 12h, deproteinization two different (first group soaked 8% NaOH 36h second treated Alcalase enzyme at concentration 0.2% 36h). Two samples we...