Alfalfa (Medicago sativa L.) is used as a high-nutrient feed for animals. Weeds are significant challenge that affects alfalfa production. Although weeds unevenly distributed, herbicides broadcast-applied in fields. In this research, object detection convolutional neural networks, including Faster R-CNN, VarifocalNet (VFNet), and You Only Look Once Version 3 (YOLOv3), were to indiscriminately d...

Medicago sativa (alfalfa) is a widely used animal feed. However, its quality has been difficult to control due the lack of appropriate marker compounds. Therefore, it very necessary select an (Q-marker) quality. In this study, medium-pressure liquid chromatography and high-pressure were employed effectively prepare separation Q-marker (L-tryptophan) from sativa. Firstly, using MCI GEL® CHP20P a...

Glyphosate-tolerant alfalfa (GTA) was developed to withstand over-the-top applications of glyphosate, the active ingredient in Roundup agricultural herbicides. As a part of the safety assessment, GTA (designated J101 x J163) was grown under controlled field conditions at geographically diverse locations within the United States during the 2001 and 2003 field seasons along with control and other...

Access to livestock manure is increasing in semi-arid cropping systems of U.S. Northwest prompting evaluation fertilization strategies on regional production systems. A study conducted from fall 2012 2019 used: fall- or spring-applied dairy (56 Mg ha−1), fall-applied composted (33 urea SUPERU (Koch Agronomic Services), and a control (n = 6) corn (Zea mays L.)–barley (Hordeum vulgare L.)–alfalfa...

Leaf area measurements in the field are very difficult to determine yield. Use of mobile application has come fore due its practical use and ease transportation. Therefore, this study, accuracy were tested by determining leaf with an automatic meter a called 'PETIOLE'. The alfalfa plants (Medicago sativa L. cv Nimet) grown field, measured 'PETIOLE' laboratory conditions automatically measuremen...

The fermentation product of herbs by lactic acid bacteria (FHL) was assayed for antifungal activities against Rosellinia necatrix, Helicobasidium mompa, Fusarium oxysporum, Pythium graminicola and Pyricularia oryzae. FHL completely inhibited the growth of R. necatrix, H. mompa, P. graminicola and P. oryzae, and reduced the growth of F. oxysporum by 35%. When the seeds of Medicago sativa L. (alf...

Peroxidase (POD) and superoxide dismutase (SOD) enzyme activities were analyzed in non-regenerative transformed embryogenic lines of alfalfa (Medicago sativa L.) carrying wound-inducible oryzacystatin I (OC-I), wound-inducible oryzacystatin I antisense (OC-Ias), or hygromycin phosphotransferase (hpt) genes. All of the transformed lines analyzed had elevated levels of all POD isoforms. Three POD...

The proteome responses to heat stress have not been well understood. In this study, alfalfa (Medicago sativa L. cv. Huaiyin) seedlings were exposed to 25 °C (control) and 40 °C (heat stress) in growth chambers, and leaves were collected at 24, 48 and 72 h after treatment, respectively. The morphological, physiological and proteomic processes were negatively affected under heat stress. Proteins ...

Seeds of alfalfa (Medicago sativa) and white clover (Trifolium repens) were primed in 3 priming solutions [2% NaCl, 2% KNO3 and 300 g/L polyethylene glycol (PEG)] for 2 days at 20°C and their subsequent germination compared with that of untreated seed. Four replications of 50 seeds for germination and 25 seeds for emergence were arranged in a 2-factor factorial completely randomised design. All...

The production of somatic embryos in alfalfa (Medicago sativa L., cv Regen S) is increased 5- to 10-fold by alanine and proline. However, utilization of nitrogen for synthesis of protein from alanine, proline, glutamate, and glycine is not qualitatively different, even though the latter two amino acids do not increase somatic embryo formation. These determinations were made by (15)N labeling wi...