The Soret absorption band of the ferrous carbon monoxide (CO) complex of cytochrome c peroxidase exhibited a blue shift from 423.7 to 420 nm upon an increase in pH from 6.5 to 8.5. The spectral change was reversible with an isosbestic point at 422 nm. The pH dependence of this spectral change gave a sigmoidal curve fitted well to a theoretical curve of a cooperative release of two protons with ...

introduction: salivary calcium and phosphate levels might have a relationship with dental caries severity. evaluation of different indexes such as alkaline phosphatase, which increases salivary phosphate levels, and hydroxyapatite ion activity, which reflects salivary calcium and phosphate levels, might help determine the risk of dental caries. the aim of the present study was to compare saliva...

The Neoproterozoic Nagarparkar Complex is characterized by isolated semicircular hillocks of outcrops of “withinplate” A-type peralkaline to peraluminous granites. Geochemically the granites/rhyolites of the Nagarparkar Complex are characterized by high SiO2, Na2O+K2O, K2O, Fe/Mg, Al2O3, Zr, Nb and Y and low CaO and Sr. Majority of samples show moderate iron enrichment. The rocks show the chara...

conclusions the elevation of hmw-alp activity in cu treated animal suggests the occurrence of biliary disease. this may be used as a biomarker for the diagnosis of copper toxicity. results obtained data showed that with increasing administration of copper, the alp activity was elevated significantly. in comparison with the control group the elevations were between 20%-56% using gel filtration c...

This communication introduces a simple method to determine the pKs of microscopic ionizations from complex titration curves. We used this approach to study the alkaline transition (pH-dependent ligand exchange) of mitochondrial cytochrome c. The linearization of titration curves permitted resolution of two to three limiting microscopic ionizations. By combining these data with studies of the te...

An alkaline phosphatase-bioreactive probe, in which the enzyme is covalently bound to the mass spectrometry target, has been developed for studies of phosphoproteins. The bioreactive probe was used in combination with affinity capture and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to study hydrolysis of several phosphoproteins found in human saliva. Human saliv...

This review article summarizes the recent progress in dental applications of natural products. Catechin gel showed selective antimicrobial activity, whereas the alkaline extract of various plant species rich in lignin carbohydrate complex (LCC) showed much higher antiviral activity than lower molecular weight polyphenols. Mouthwash with the alkaline extract of a plant classified as OTC effectiv...