Alkylation reactions of the nucleoside guanosine (Guo) by the α,β-unsaturated compounds (α,β-UC) acrylonitrile (AN), acrylamide (AM), acrylic acid (AA) and acrolein (AC), which can act as alkylating agents of DNA, were investigated kinetically. The following conclusions were drawn: i) The Guo alkylation mechanism by AC is different from those brought about the other α,β-UC; ii) for the first th...

Escherichia coli can ameliorate the toxic effects of alkylating agents either by preventing DNA alkylation or by repairing DNA alkylation damage. The alkylation-sensitive phenotype of E. coli alkB mutants marks the alkB pathway as an extremely effective defense mechanism against the cytotoxic effects of the SN2, but not the SN1, alkylating agents. Although it is clear that AlkB helps cells to b...

Eeny, meeny, miny ... enaminones! Lactams and imides have been shown to consistently provide enantioselectivities substantially higher than other substrate classes previously investigated in the palladium-catalyzed asymmetric decarboxylative allylic alkylation. Several new substrates have been designed to probe the contributions of electronic, steric, and stereoelectronic factors that distingui...

Engineering of hybrid gene clusters between the toluene metabolic tod operon and the biphenyl metabolic bph operon greatly enhanced the rate of biodegradation of trichloroethylene. Escherichia coli cells carrying a hybrid gene cluster composed of todC1 (the gene encoding the large subunit of toluene terminal dioxygenase in Pseudomonas putida F1), bphA2 (the gene encoding the small subunit of bi...

The mol-ecule of the title compund, C(30)H(28)N(2)O(2), a Schiff base synthesised via a condensation reaction between 4-meth-oxy-benzaldehyde and 3,3'-dimethyl-benzidine, a crystallographic twofold rotation axis passes through the mid-point of the C-C bond of the biphenyl unit. Thus, the asymmetric unit comprises one half-mol-ecule. In the biphenyl unit, the aromatic rings are twisted by 13.49 ...

In this work, biphenyl and biphenyl oxide were extracted by direct single drop microextraction (direct-SDME) and analyzed by gas chromatography flame ionization detection. The extraction occurred by suspending a 7 μL drop of toluene (as extracting solvent) containing acetonaphton (as internal standard) from the tip of a microsyringe in direct-SDME, respectively. The effect of different paramete...

Rhodococcus sp. RHA1 induces two biphenyl dioxygenases, the BphA and EtbA/EbdA dioxygenases, during growth on biphenyl. Their subunit genes were expressed in R. erythropolis IAM1399 to investigate the involvement of each subunit gene in their activity and their substrate preferences. The recombinant expressing ebdA1A2A3etbA4 and that expressing bphA1A2A3A4 exhibited 4-chlorobiphenyl (4-CB) tran...

Aims: To synthesize small molecule alkylating compounds and analyze the kinetics of the alkylation in aqueous solution. Determine molecular properties and the drug likeness of these four compounds as potential antineoplastic agents and apply statistical analysis to identify interrelationships of properties. Study Design: Four compounds were synthesized, characterized, and studied for alkylation...

The title compound, C(14)H(10)Cl(4)O(4)S, is a 2,2,2-trichloro-ethyl-protected precursor of 4'-chloro-biphenyl-3-yl sulfate, a sulfuric acid ester of 4'-chloro-biphenyl-3-ol. The C(aromatic)-O and O-S bond lengths of the C(aromatic)-O-S unit are comparable to those in structurally analogous biphenyl-4-yl 2,2,2-trichloro-ethyl sulfates with no electro-negative chlorine substituent in the benzene...