α-Amylase from Anoxybacillus thermarum A4 was purified using ammonium sulphate precipitation and Sephadex G-100 gel filtration chromatography, with 29.8-fold purification and 74.6% yield. A4 amylase showed best performance for soluble potato starch hydrolysis at 70 °C and pH 5.5-10.5. A4 amylase was extremely stable at +4 °C, and the enzyme retained over 65% of its original α-amylase activity a...

Abstract. Maser WH, Maiyah N, Nagarajan M, Kingwascharapong P, Senphan T, Ali AMM, Bavisetty SCB. 2023. Effect of different extraction solvents on the yield and enzyme inhibition (a-amylase, a-glucosidase, lipase) activity some vegetables. Biodiversitas 24: 3320-3331. The present study investigated in vitro a-amylase, lipase, antioxidant activities (DPPH, metal chelating, FRAP) Allium cepa L., ...

Gibberellic acid (GA) enhances the synthesis otf a-amylase andl ribonutclease in isolated aleurone layers andl this process is inhibited by abscisiin. Removal of gibberellic acid in mid-course of a-amylase productioni results in a slowing down of a-amylase synthesis, suggesting a continued requirement of GA for eiizyme synthesis. This is paralleled by a continuous requirement for RNA synthesis....

The aim of this work was to evaluate the inhibitory activities of methanolic extracts of Artocarpus altilis, Cinnamomum zeylanicum, Piper betel and Artocarpus heterophyllus on Wheat alpha amylase and Baker’s yeast alpha glucosidase at varying concentrations. Diabetes mellitus is a clinical condition characterized by hyperglycaemia in which an elevated amount of glucose circulates in the blood p...

background and objectives: salvia chloroleuca rech. f. & allen., is an endemic species growing wildly in north east and center parts of iran but there is no information about its safety. to provide information about the safety of the species, we evaluated its acute and sub chronic toxicity in rats. methods: in acute toxicity study, the aqueous methanol (80%) extract at a single dose of 2000...

potato tuberworm, phthorimaea operculella zeller (lepidoptera; gelechiidae) is a worldwide pest of solanaceous crops. larvae feed inside galleries in foliage, stems and tubers making chemical control unsuccessful, so other control methods should be applied. in recent years many plants have received genes that encode toxic proteins as a strategy to resist insect pests. in this study, optimal ph ...

The study aimed to investigate extracts of un-ripe pods Vinca rosea cv. Pink for antidiabetic activity and polyphenol-based characterization using HPLC. Moreover, the molecular interaction identified markers with targets was explored molecular-docking software. Different were prepared investigated models such as glucose uptake by yeast cells, alpha amylase inhibition haemoglobin-glycosylation a...

The effect of triticale α-amylases inhibitors on starch hydrolysis catalyzed by the Sunn pest, Eurygaster integriceps Puton (Hemiptera: Scutelleridae) midgut amylases was examined. Biochemical studgawies showed that inhibitors from Triticale (a hybrid of wheat and rye) had inhibitiory effects on E. integriceps α-amylases. The effects of the triticale α-amylase inhibitor (T-αAI) on α-amylase of ...

The antioxidant properties and effect of essential oil of black pepper (Piper guineense) seeds on α -amylase, α -glucosidase (key enzymes linked to type-2 diabetes), and angiotensin-I converting enzyme (ACE) (key enzyme linked to hypertension) were assessed. The essential oil was obtained by hydrodistillation and dried with anhydrous Na2SO4, and the phenolic content, radical [1,1-diphenyl-2 pic...

Background and objectives: Salvia chloroleuca Rech. f. & Allen., is an endemic species growing wildly in north east and center parts of Iran but there is no information about its safety. To provide information about the safety of the species, we evaluated its acute and sub chronic toxicity in rats. Methods: In acute toxicity study, the aqueous methanol (80%) extract at a single dose of...