This study was focused on purification and characterization of keratinase from Bacillus thuringiensis TS2, isolated from feather dumping soil site. Zymogram of crude enzyme on native-PAGE presented bands with keratinase activity of molecular weights is 41 kDa. In purification steps 2.69 fold purification was achieved after 80% ammonium precipitation of the with 34.42% recovery. In further purif...

The membrane protein complex translocase mediates the translocation of bacterial proteins. In this complex, the SecY, SecE, and SecG proteins constitute an integral membrane domain. Sequence comparison revealed a potential secG-like gene in the gram-positive soil bacterium Streptomyces lividans. Chromosomal deletion of this gene resulted in a sporulation defect and an overall deficiency in secr...

Pectinases are industrially important enzymes. The aim of this work was the isolation of pectinases producing Bacillus species from soil sample and their identification by phenotypic tests as well as production and purification pectinases enzyme. Isolated bacteria Subjected to various screening activity and gives pectinases screening test positive i.e. bacteria produced pectinases enzyme. The e...

The objectives of this paper were to determine the response of soil quality indicators and organic carbon (OC) distributions within different aggregate classes to changes in land use from desert soils to cropland occurring in the Abarkooh plain, Central Iran. Composite soil samples of the desert soil, wheat and alfalfa fields were taken from three different depths and soil quality indicators, i...

background: screening is a routine procedure for isolation of microorganisms which are able to produce special metabolites. purified thermostable α-amylase from bacterial sources is widely used in different industries. in this study we analyzed samples collected from three different hot springs in iran to detect any strains capable of producing thermostable α-amylase. materials and methods: hot...

Owing to the production of alpha, beta and gamma amylase subtypes; starch degrading microbes, especially bacteria have an invincible role in the food, fermentation, textile and paper industries. Of them, α-amylases from Bacillus spp. have contributed tremendous advancements in bio-industry, especially in starch, detergent and pharmaceutical arena. Though general reviews are seen in literature o...