The dynamics of mesophilic and thermophilic bacterial population was studied in the bulk soil and rhizosphere of the grass Aristida adscensionis L. in a caatinga of the semi-arid Brazilian Northeast. Mesophilic heterotrophic bacteria, spore–forming bacteria and actinomycetes were significantly more abundant than thermophiles, and their counts were 10 10 CFU g dry soil, showing weak fluctuations...

Zabrotes subfasciatus larvae possess three alpha-amylase isoforms as determined by in gel assays following SDS-PAGE. The two minor isoforms present lower electrophoretic mobility than the major form, and seem to occur as a heterodimer. When developed inside Vigna unguiculata (cowpea) seeds, fourth instar larvae have minor quantities of the slow-migrating forms, but when reared on seeds of Phase...

Investigations were made concerning the number and properties of amylase in sugarcane leaves. Enzymes were extracted froin leaf samples which had been frozen, lyophilized, and ground to a fine powder. Techniques of differential solubihty, gel filtiation, and papei electrophoresis were employed to remove amylase from the crude extracts. Solubility studies showed that amylase was moderately preci...

While diversity studies and screening for enzyme activities are important elements of understanding fungal roles in the soil ecosystem, extracting purifying target from cellular system is also required to characterize enzyme. This is, particular, necessary before developing industrial-scale production. In present study, partially purified α-amylase was obtained strains Pseudogymnoascus sp. Anta...

Amylases are key enzymes in the processing of starch many kingdoms life. They important catalysts industrial biotechnology where they applied in, among others, food and production detergents. In man amylases first digestion to glucose arguably also preferred target therapeutic strategies aimed at treatment type 2 diabetes patients through down-tuning assimilation. Efficient sensitive assays tha...

The manufacturing of ethanol from cereal crop by enzymatic conversion of starch to fermentable glucose and fermentation by yeast is now a well established process. The first phase of the process is the addition of alpha-amylases to the liquefaction of the milled grains. Alpha-amylases hydrolyses the α-1,4-glucosidic bonds to reduce the viscosity of gelatinized starch producing soluble starch an...

Fungal amylolytic enzymes, including α-amylase, gluocoamylase and α-glucosidase, have been extensively exploited in diverse industrial applications such as high fructose syrup production, paper making, food processing and ethanol production. In this paper, amylolytic genes of 85 strains of fungi from the phyla Ascomycota, Basidiomycota, Chytridiomycota and Zygomycota were annotated on the genom...

Starch is the major source of carbohydrates in the human diet. Starch is present in many different fruits, vegetables, roots, and grains. Starch and starch derivatives are a nutritive, abundant, and economical food source. Starch can be consumed unprocessed in the form of raw fruits and vegetables or in the form of more shelf-stable processed foods. Food starches contribute to the characteristi...