The aim of this study is to identify the effects KDML105 bran extract on gene expression involving hair cycle in HFDPCs and investigate its bioactive constituents, antioxidant, anti-inflammatory activities. content tocopherols, γ-oryzanol, phytic acid, phenolic compounds was quantified by liquid chromatography. Free fatty acids were determined using gas Antioxidant capacities estimated DPPH, AB...

[Background and Objective] Hair cycle is a highly regulated cyclical process. In this cycle, three phases were defined; anagen (growing phase), catagen (regressing phase) telogen (resting phase). Hypertrichosis well-known side effect in patients receiving an immunosuppressant, cyclosporin A (CsA). CsA also elongates organ culture system of human hair follicles. Therefore, thought to cause hyper...

In contrast to the dynamic and striking changes in hair follicle (HF) morphology during the anagen and catagen phases of the hair cycle, the telogen follicle appears static. However, it has been argued that the quiescent appearance of telogen HFs is deceptive (Davis 1962, Stenn, Paus 2001, Higgins, Westgate 2009). For example, the expression of some genes clearly peaks during telogen (Greco, Ch...

r-phycoerythrin (r-pe), a fluorescent protein from phycobiliprotein family, is isolated from red algae. conjugation of antibodies to r-pe facilitates multiple fluorescent staining methods. in the present study polyclonal antibodies and polyclonal f(ab')2 fragment antibodies were conjugated to r-pe by two different methods. the efficiency of the methods was evaluated using immunocytochemistry (i...

Hair follicle formation depends on reciprocal epidermal-dermal interactions and occurs during skin development, but not in adult life. This suggests that the properties of dermal fibroblasts change during postnatal development. To examine this, we used a PdgfraEGFP mouse line to isolate GFP-positive fibroblasts from neonatal skin, adult telogen and anagen skin and adult skin in which ectopic ha...

The dermal sheath (DS) of the hair follicle is comprised by fibroblast-like cells and extends along the follicular epithelium, from the bulb up to the infundibulum. From this structure, cells with stem characteristics were isolated: they have a mesenchymal origin and express CD90 protein, a typical marker of mesenchymal stem cells. It is not yet really clear in which region of hair follicle the...

Estrogen dysregulation causes hair disorder. Clinical observations have demonstrated that estrogen raises the telogen/anagen ratio and inhibits hair shaft elongation of female scalp hair follicles. In spite of these clinical insights, the properties of estrogen on hair follicles are poorly dissected. In the present study, we show that estrogen induced apoptosis of precortex cells and caused pre...

Acquiring induced pluripotent stem cells (IPSC) lines from probands with rare diseases allows investigating properties of difficult to obtain tissues. We obtained IPSC dermal fibroblasts two patients recessive form dystrophic epidermolysis bullosa (RDEB) using the kit Sendai virus mediated gene transfer. Patients have different mutation types in COL7A1 and RDEB forms. approved IPSCs status expr...