Testosterone-estradiol-binding globulin in human plasma was characterized and quantified by polyacrylamide gel electrophoresis. Testosterone and estradiol were shown to bind to a single homogeneous protein over a wide range of experimental conditions. Testosterone binding to cortisolbinding globulin (transcortin) and estradiol binding to albumin were clearly demonstrated; both transcortin and a...

We propose in the present paper to describe a system of lipoproteins which contain phospholipid but no free cholesterol and little or no esterified cholesterol. We utilize data which we have accumulated during the past four years during which time we have studied serum proteins and lipids by making multiple analyses of ten samples of each ultracentrifugate of untreated serum. The samples of cen...

BACKGROUND Dietary phytoestrogens are ligands for the estrogen receptor and may mimic estrogenic effects in vivo. OBJECTIVE To assess the biological activity of isoflavone phytoestrogens, we analyzed the effect of dietary soy isoflavone supplementation on in vivo bioassays of estrogenicity. DESIGN Fifty healthy postmenopausal women aged 50-75 y participated in a double-blind, placebo-contro...

Blood was taken from women in each trimester of pregnancy at routine antenatal visits. Further samples were collected from an age matched control group of euthyroid women taking no medication. Free triiodothyronine and free thyroxine concentrations were measured by direct radioimmunoassay (Amerlex Kit methods, Amersham International, Amersham, Buckinghamshire).2 These free hormone assays use th...

University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic (Received 1 March 2013) The aim of this study was to evaluate the serum proteins electrophoretic pattern and the concentrations of acute phase proteins in calves with chronic bronchopneumonia. Twenty-eight calves with clinical symptoms of disease were included into the evaluation. Blood serum was analyzed for total protein ...