Enzyme linked immunosorbent assay (ELISA) was applied to detect the antibody of Japanese flounder (Paralichthys olivaceus) against lymphocystis disease virus (LCDV). Purified Japanese flounder LCDV was used as a capture antigen of ELISA. Monitoring the immune response of Japanese flounder to LCDV was done using the sera obtained from apparently healthy, LCD-diseased and LCD-recovered fish. ELIS...

A 1:1 matched case-control study of calves under 1 month of age was carried out by weekly visits to 7 dairy farms in Mashhad from May 2008 to October 2008. Fecal samples were collected from a total of 112 calves with clinical signs of diarrhea and from 112 matched animals without clinical signs of diarrhea as assessed by a scoring system. The samples were investigated for the presence of Rotavi...

Bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) are orbiviruses that infect both livestock and wild ruminants. Antigenic cross-reactivity between BTV and EHDV often results in serologic misdiagnosis. Competitive enzyme-linked immunosorbent assays (c-ELISAs) show increased sensitivity and specificity for the identification of these viral diseases; however, the preparation o...

OBJECTIVE Rabies is invariably a fatal encephalomyelitis that is considered to be a serious public health problem. It is necessary to develop standard rabies virus diagnostic tools, especially for diagnosing the strains prevalent in China. METHODS Monoclonal antibodies (MAbs) specific to rabies virus were produced and characterized by enzyme linked immunosorbent assay (ELISA), isotyping, affi...

background: regarding that accurate diagnosis of human hydatidosis still needs more investigations, the present study was conducted to clone, express, and evaluate the gene encoding agb subunits (egagb16 kda) from echinococcus granulosus (iranian g1 strain) and its evaluation by elisa test. methods: dna was extracted from protoscoleces and was utilized by pcr for strain identification. total rn...

recently it has been reported, that immunoglobulin y (igy) can be used instead of polyclonal antibodies extracted from mammals (igg) for the purpose of diagnosis and therapy. these antibodies are found to have better properties in terms of specificity and ease of large-scale production. in addition, igy binds neither to mammalian complement or fc-receptors nor does it interfere with rheumatoid ...

Background: To explore the oral absorption of scorpion venom an ELISA were designed in this study. Scorpions and their venom were been used for centuries as medical treatments in traditional medicine. The oral administration of drug referred as the convenient way, as there was not any publication about gastro-intestinal absorption of scorpion venom; this experiment checked oral absorption of Me...

Orthopoxvirus species like cowpox, vaccinia and monkeypox virus cause zoonotic infections in humans worldwide. Infections often occur in rural areas lacking proper diagnostic infrastructure as exemplified by monkeypox, which is endemic in Western and Central Africa. While PCR detection requires demanding equipment and is restricted to genome detection, the evidence of virus particles can comple...