The Arabidopsis thaliana multi-stress regulator TSPO is transiently induced by abiotic stresses. The final destination of this polytopic membrane protein is the Golgi apparatus, where its accumulation is strictly regulated, and TSPO is downregulated through a selective autophagic pathway. TSPO-related proteins regulate the physiology of the cell by generating functional protein complexes. A spl...

We have used freeze-fracture electron microscopy to examine the oligomeric structure and molecular asymmetry of integral plasma membrane proteins. Recombinant plasma membrane proteins were functionally expressed in Xenopus laevis oocytes, and the dimensions of their freeze-fracture particles were analyzed. To characterize the freeze-fracture particles, we compared the particle cross-sectional a...

Reverse osmosis (RO) is a water purifcation technology that uses a semipermeable membrane to remove ions, molecules, and larger particles for the production of drinking water. The frst RO membrane for seawater desalination, wastewater treatment and other applications were made of cellulose acetate. But, the polyamide thin-flm composite membrane that can tolerate wide pH...

Aquaporins form a family of water and solute channel proteins and are present in most living organisms. In plants, aquaporins play an important role in the regulation of root water transport in response to abiotic stresses. In this work, we investigated the role of phosphorylation of plasma membrane intrinsic protein (PIP) aquaporins in the Arabidopsis thaliana root by a combination of quantita...

The importance of short membrane sequence motifs has been shown in many works and emphasizes the related sequence motif analysis. Together with specific transmembrane helix-helix interactions, the analysis of interacting sequence parts is helpful for understanding the process during membrane protein folding and in retaining the three-dimensional fold. Here we present a simple high-throughput an...

Herbaceous peony is a potential material for cut flowers, but its short vase life seriously affects the development of herbaceous flowers industry. In this study, polyphenols were applied to extend and results indicated that 8% mass concentration increased superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) activities; soluble protein content flowers; effectively reduced malondialdehyd...

In this study, novel thin-film nanocomposite (TFN) membranes were developed by incorporating graphene oxide (GO) and Aquaporin Z (AqpZ) reconstituting nanostructure (AQN) into the polyamide (PA) active layer to improve forward osmosis (FO) performances of PA TFN membranes. First, AQN loading in was optimized, followed GO addition at various loadings until optimal FO process performance attained...

The chromosome location of the gene encoding aquaporin 3 (AQP3), which functions as a channel for water and small polar solutes in the basolateral membrane of the collecting duct of the kidney, was determined. In situ hybridization on metaphase chromosomes allowed the assignment of human AQP3 to chromosome 9p21-->p12.

(1) Background: The unusual accumulation of Na,K-ATPase complexes in the brush border membrane choroid plexus epithelial cells have intrigued researchers for decades. However, full range expressed subunits and their relation to microvillus cytoskeleton remains unknown. (2) Methods: RT-PCR analysis, co-immunoprecipitation, native PAGE, mass spectrometry, differential centrifugation were combined...

Background: Considering the carcinogenic effects of heavy metals, such as chromium, it is essential to remove these elements from water and wastewater. Direct osmosis is a new membrane technology, which can be a proper alternative to conventional chromium removal processes. Methods: The wastewater samples were collected from an electroplating unit, located in Alborz industrial city, Qazvin, Ir...