In this work, the recovery and purification of inulinase produced by Kluyveromyces marxianus NRRL Y-7571 in solid state fermentation using in aqueous two-phase systems (ATPS) was studied. The effects of concentration of PEG and potassium phosphate were evaluated using experimental design technique. The SAB extraction yielded a purification factor (PF) up to 3.85-fold, using 18% PEG 4000 and 7% ...

In this paper electrical conductivity measurements and optical investigations takes place in AOT/ heptane/ (water or aqueous) systems. The water in oil system prepared with adding water in 50% by weight of AOT/ heptane solution.  In other systems instead of water, aqueous solutions of 1) zinc acetate and water 2) Potassium Hydroxide and water were used. In each phase, the trend of the electrica...

A relationship is proposed for the interfacial partitioning of protein in poly(ethylene glycol) (PEG)-phosphate aqueous two-phase system (ATPS). The relationship relates the natural logarithm of interfacial partition coefficient, ln G to the PEG concentration difference between the top and bottom phases, Δ[PEG], with the equation ln G=AΔ[PEG]+B. Results showed that this relationship provides go...

BACKGROUND Microalgae can synthesize starch with productivity higher than conventional terrestrial crops, without the need for arable land. However, little is known about processes to extract from microalgae. Here, a biorefinery process described including microalgal cell disruption followed by extraction of and pigments aqueous two-phase system (ATPS) using choline chloride polypropylene glyco...

The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) prot...

Papain was purified from spray-dried Carica papaya latex using aqueous two-phase system (ATPS). Then it was recovered from PEG phase by in situ immobilization or preparing cross-linked enzyme aggregates (CLEAs). The Plackett-Burman design and the central composite design (CCD) together with the response surface methodology (RSM) were used to optimize the APTS processes. The highly purified papa...

The interference of some specific aqueous two-phase system (ATPS) phase-forming components in bovine serum albumin (BSA) determination by the Bradford method was investigated. For this purpose, calibration curves were obtained for BSA in the presence of different concentrations of salts and polymers. A total of 19 salts [Na₂SO₄, (NH₄)₂SO₄, MgSO₄, LiSO₄, Na₂HPO₄, sodium phosphate buffer (pH 7.0)...